In Vivo Labeling by CD73 Marks Multipotent Stromal Cells and Highlights Endothelial Heterogeneity in the Bone Marrow Niche

Breitbach M, Kimura K, Luis TC, Fuegemann CJ, Woll PS, Hesse M, Facchini R, Rieck S, Jobin K, Reinhardt J, Ohneda O, Wenzel D, Geisen C, Kurts C, Kastenmueller W, Hoelzel M, Jacobsen SEW, Fleischmann BK (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Cell Stem Cell Elsevier (Cell Press)

Book Volume: 22

Pages Range: 262-276.e7

Journal Issue: 2

DOI: 10.1016/j.stem.2018.01.008

Abstract

Despite much work studying ex vivo multipotent stromal cells (MSCs), the identity and characteristics of MSCs in vivo are not well defined. Here, we generated a CD73-EGFP reporter mouse to address these questions and found EGFP + MSCs in various organs. In vivo, EGFP + mesenchymal cells were observed in fetal and adult bones at proliferative ossification sites, while in solid organs EGFP + cells exhibited a perivascular distribution pattern. EGFP + cells from the bone compartment could be clonally expanded ex vivo from single cells and displayed trilineage differentiation potential. Moreover, in the central bone marrow CD73-EGFP + specifically labeled sinusoidal endothelial cells, thought to be a critical component of the hematopoietic stem cell niche. Purification and molecular characterization of this CD73-EGFP + population revealed an endothelial subtype that also displays a mesenchymal signature, highlighting endothelial cell heterogeneity in the marrow. Thus, the CD73-EGFP mouse is a powerful tool for studying MSCs and sinusoidal endothelium. Breitbach et al. generated a CD73-EGFP reporter mouse that enabled identification and tracking of multipotent stromal cells in vivo. CD73-EGFP also labeled sinusoidal endothelial cells within the bone marrow, enabling the molecular characterization of this important endothelial component of the hematopoietic stem cell niche.

Involved external institutions

Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE) University of Oxford GB United Kingdom (GB) University of Tsukuba / 筑波大学 JP Japan (JP)

How to cite

APA:

Breitbach, M., Kimura, K., Luis, T.C., Fuegemann, C.J., Woll, P.S., Hesse, M.,... Fleischmann, B.K. (2018). In Vivo Labeling by CD73 Marks Multipotent Stromal Cells and Highlights Endothelial Heterogeneity in the Bone Marrow Niche. Cell Stem Cell, 22(2), 262-276.e7. https://dx.doi.org/10.1016/j.stem.2018.01.008

MLA:

Breitbach, Martin, et al. "In Vivo Labeling by CD73 Marks Multipotent Stromal Cells and Highlights Endothelial Heterogeneity in the Bone Marrow Niche." Cell Stem Cell 22.2 (2018): 262-276.e7.

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