Neuroimmunology: Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation
Costa Jordao MJ, Sankowski R, Brendecke SM, Sagar , Locatelli G, Tai YH, Tay TL, Schramm E, Armbruster S, Hagemeyer N, Gross O, Mai D, Cicek O, Falk T, Kerschensteiner M, Grun D, Prinz M (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 363
Article Number: eaat7554
Journal Issue: 6425
Abstract
The innate immune cell compartment is highly diverse in the healthy central nervous system (CNS), including parenchymal and non-parenchymal macrophages. However, this complexity is increased in inflammatory settings by the recruitment of circulating myeloid cells. It is unclear which disease-specific myeloid subsets exist and what their transcriptional profiles and dynamics during CNS pathology are. Combining deep single-cell transcriptome analysis, fate mapping, in vivo imaging, clonal analysis, and transgenic mouse lines, we comprehensively characterized unappreciated myeloid subsets in several CNS compartments during neuroinflammation. During inflammation, CNS macrophage subsets undergo self-renewal, and random proliferation shifts toward clonal expansion. Last, functional studies demonstrated that endogenous CNS tissue macrophages are redundant for antigen presentation. Our results highlight myeloid cell diversity and provide insights into the brain's innate immune system.
Involved external institutions
Albert-Ludwigs-Universität Freiburg
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
How to cite
APA:
Costa Jordao, M.J., Sankowski, R., Brendecke, S.M., Sagar, ., Locatelli, G., Tai, Y.-H.,... Prinz, M. (2019). Neuroimmunology: Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation. Science, 363(6425). https://doi.org/10.1126/science.aat7554
MLA:
Costa Jordao, Marta Joana, et al. "Neuroimmunology: Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation." Science 363.6425 (2019).
BibTeX: Download