Systematic Identification of Cell-Cell Communication Networks in the Developing Brain
Sheikh BN, Bondareva O, Guhathakurta S, Tsang TH, Sikora K, Aizarani N, Sagar , Holz H, Gruen D, Hein L, Akhtar A (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 21
Pages Range: 273-287
DOI: 10.1016/j.isci.2019.10.026
Abstract
Since the generation of cell-type specific knockout models, the importance of inter-cellular communication between neural, vascular, and microglial cells during neural development has been increasingly appreciated. However, the extent of communication between these major cell populations remains to be systematically mapped. Here, we describe EMBRACE (embryonic brain cell extraction using FACS), a method to simultaneously isolate neural, mural, endothelial, and microglial cells to more than 94% purity in ∼4 h. Utilizing EMBRACE we isolate, transcriptionally analyze, and build a cell-cell communication map of the developing mouse brain. We identify 1,710 unique ligand-receptor interactions between neural, endothelial, mural, and microglial cells in silico and experimentally confirm the APOE-LDLR, APOE-LRP1, VTN-KDR, and LAMA4-ITGB1 interactions in the E14.5 brain. We provide our data via the searchable “Brain interactome explorer”, available at https://mpi-ie.shinyapps.io/braininteractomeexplorer/. Together, this study provides a comprehensive map that reveals the richness of communication within the developing brain.
Involved external institutions
Albert-Ludwigs-Universität Freiburg
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
How to cite
APA:
Sheikh, B.N., Bondareva, O., Guhathakurta, S., Tsang, T.H., Sikora, K., Aizarani, N.,... Akhtar, A. (2019). Systematic Identification of Cell-Cell Communication Networks in the Developing Brain. iScience, 21, 273-287. https://doi.org/10.1016/j.isci.2019.10.026
MLA:
Sheikh, Bilal N., et al. "Systematic Identification of Cell-Cell Communication Networks in the Developing Brain." iScience 21 (2019): 273-287.
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