Mapping microglia states in the human brain through the integration of high-dimensional techniques
Sankowski R, Bottcher C, Masuda T, Geirsdottir L, Sagar , Sindram E, Seredenina T, Muhs A, Scheiwe C, Shah MJ, Heiland DH, Schnell O, Grun D, Priller J, Prinz M (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 22
Pages Range: 2098-2110
Journal Issue: 12
DOI: 10.1038/s41593-019-0532-y
Abstract
Microglia are tissue-resident macrophages of the CNS that orchestrate local immune responses and contribute to several neurological and psychiatric diseases. Little is known about human microglia and how they orchestrate their highly plastic, context-specific adaptive responses during pathology. Here we combined two high-dimensional technologies, single-cell RNA-sequencing and time-of-flight mass cytometry, to identify microglia states in the human brain during homeostasis and disease. This approach enabled us to identify and characterize a previously unappreciated spectrum of transcriptional states in human microglia. These transcriptional states are determined by their spatial distribution, and they further change with aging and brain tumor pathology. This description of multiple microglia phenotypes in the human CNS may open promising new avenues for subset-specific therapeutic interventions.
Authors with CRIS profile
Involved external institutions
Albert-Ludwigs-Universität Freiburg
Germany (DE)
Charité - Universitätsmedizin Berlin
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
AC Immune
Switzerland (CH)
Germany (DE)
Charité - Universitätsmedizin Berlin
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
AC Immune
Switzerland (CH)
How to cite
APA:
Sankowski, R., Bottcher, C., Masuda, T., Geirsdottir, L., Sagar, ., Sindram, E.,... Prinz, M. (2019). Mapping microglia states in the human brain through the integration of high-dimensional techniques. Nature Neuroscience, 22(12), 2098-2110. https://doi.org/10.1038/s41593-019-0532-y
MLA:
Sankowski, Roman, et al. "Mapping microglia states in the human brain through the integration of high-dimensional techniques." Nature Neuroscience 22.12 (2019): 2098-2110.
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