Heterogeneity of immune cells in human atherosclerosis revealed by scRNA-Seq

Vallejo J, Cochain C, Zernecke A, Ley K (2021)

Publication Type: Journal article, Review article

Publication year: 2021

Journal

Cardiovascular Research Oxford University Press

Book Volume: 117

Pages Range: 2537-2543

Journal Issue: 13

DOI: 10.1093/cvr/cvab260

Abstract

Immune cells in atherosclerosis include T, B, natural killer (NK) and NKT cells, macrophages, monocytes, dendritic cells (DCs), neutrophils, and mast cells. Advances in single-cell RNA sequencing (sRNA-Seq) have refined our understanding of immune cell subsets. Four recent studies have used scRNA-Seq of immune cells in human atherosclerotic lesions and peripheral blood mononuclear cells (PBMCs), some including cell surface phenotypes revealed by oligonucleotide-tagged antibodies, which confirmed known and identified new immune cell subsets and identified genes significantly up-regulated in PBMCs from HIV+ subjects with atherosclerosis compared to PBMCs from matched HIV+ subjects without atherosclerosis. The ability of scRNA-Seq to identify cell types is greatly augmented by adding cell surface phenotype using antibody sequencing. In this review, we summarize the latest data obtained by scRNA-Seq on plaques and human PBMCs in human subjects with atherosclerosis.

Involved external institutions

La Jolla Institute for Immunology US United States (USA) (US) Universitätsklinikum Würzburg DE Germany (DE)

How to cite

APA:

Vallejo, J., Cochain, C., Zernecke, A., & Ley, K. (2021). Heterogeneity of immune cells in human atherosclerosis revealed by scRNA-Seq. Cardiovascular Research, 117(13), 2537-2543. https://doi.org/10.1093/cvr/cvab260

MLA:

Vallejo, Jenifer, et al. "Heterogeneity of immune cells in human atherosclerosis revealed by scRNA-Seq." Cardiovascular Research 117.13 (2021): 2537-2543.

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