Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation
Probst S, Sagar , Tosic J, Schwan C, Grun D, Arnold SJ (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 148
Article Number: dev193789
Journal Issue: 1
DOI: 10.1242/dev.193789
Abstract
Anterior mesoderm (AM) and definitive endoderm (DE) progenitors represent the earliest embryonic cell types that are specified during germ layer formation at the primitive streak (PS) of the mouse embryo. Genetic experiments indicate that both lineages segregate from Eomes-expressing progenitors in response to different Nodal signaling levels. However, the precise spatiotemporal pattern of the emergence of these cell types and molecular details of lineage segregation remain unexplored. We combined genetic fate labeling and imaging approaches with single-cell RNA sequencing (scRNA-seq) to follow the transcriptional identities and define lineage trajectories of Eomes-dependent cell types. Accordingly, all cells moving through the PS during the first day of gastrulation express Eomes. AM and DE specification occurs before cells leave the PS from Eomes-positive progenitors in a distinct spatiotemporal pattern. ScRNA-seq analysis further suggested the immediate and complete separation of AM and DE lineages from Eomes-expressing cells as last common bipotential progenitor.
Involved external institutions
Albert-Ludwigs-Universität Freiburg
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
Germany (DE)
Max-Planck-Institut für Immunbiologie und Epigenetik (MPI-IE) / Max Planck Institute of Immunobiology and Epigenetics
Germany (DE)
How to cite
APA:
Probst, S., Sagar, ., Tosic, J., Schwan, C., Grun, D., & Arnold, S.J. (2021). Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation. Development, 148(1). https://dx.doi.org/10.1242/dev.193789
MLA:
Probst, Simone, et al. "Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation." Development 148.1 (2021).
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