3D printed neural tissues with in situ optical dopamine sensors
Li J, Reimers A, Dang KM, Brunk MG, Drewes J, Hirsch UM, Willems C, Schmelzer CE, Groth T, Nia AS, Feng X, Adelung R, Sacher WD, Schütt F, Poon JK (2023)
Publication Type: Journal article
Publication year: 2023
Journal
Book Volume: 222
Article Number: 114942
DOI: 10.1016/j.bios.2022.114942
Abstract
Engineered neural tissues serve as models for studying neurological conditions and drug screening. Besides observing the cellular physiological properties, in situ monitoring of neurochemical concentrations with cellular spatial resolution in such neural tissues can provide additional valuable insights in models of disease and drug efficacy. In this work, we demonstrate the first three-dimensional (3D) tissue cultures with embedded optical dopamine (DA) sensors. We developed an alginate/Pluronic F127 based bio-ink for human dopaminergic brain tissue printing with tetrapodal-shaped-ZnO microparticles (t-ZnO) additive as the DA sensor. DA quenches the autofluorescence of t-ZnO in physiological environments, and the reduction of the fluorescence intensity serves as an indicator of the DA concentration. The neurons that were 3D printed with the t-ZnO showed good viability, and extensive 3D neural networks were formed within one week after printing. The t-ZnO could sense DA in the 3D printed neural network with a detection limit of 0.137 μM. The results are a first step toward integrating tissue engineering with intensiometric biosensing for advanced artificial tissue/organ monitoring.
Involved external institutions
Max-Planck-Institut für Mikrostrukturphysik (MSP) / Max Planck Institute for Microstructure Physics
Germany (DE)
Martin-Luther-Universität Halle-Wittenberg (MLU)
Germany (DE)
Fraunhofer-Institut für Mikrostruktur von Werkstoffen und Systemen IMWS
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
Germany (DE)
Martin-Luther-Universität Halle-Wittenberg (MLU)
Germany (DE)
Fraunhofer-Institut für Mikrostruktur von Werkstoffen und Systemen IMWS
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
How to cite
APA:
Li, J., Reimers, A., Dang, K.M., Brunk, M.G., Drewes, J., Hirsch, U.M.,... Poon, J.K. (2023). 3D printed neural tissues with in situ optical dopamine sensors. Biosensors and Bioelectronics, 222. https://dx.doi.org/10.1016/j.bios.2022.114942
MLA:
Li, Jianfeng, et al. "3D printed neural tissues with in situ optical dopamine sensors." Biosensors and Bioelectronics 222 (2023).
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