Deliolanis NC, Ntziachristos V (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 8
Pages Range: 438-443
Journal Issue: 5
Fluorescence molecular tomography of tissues is a method that three-dimensionally resolves fluorescence biodistribution in vivo, with applications in small-animal research and pre-clinical diagnostics. There are many alternative imaging geometries in optical tomographic experimental systems, but in general, all imaging setups consist of four subsystems: illumination, animal mount, imaging, and automation and data acquisition (i.e., electronics and computer). Here we refer to charge-coupled device (CCD)-based systems that work in trans-illumination (i.e., illumination and detection occur on opposite sides of the subject), while a mouse or other small animal is rotated through 360° to allow photon acquisition from multiple projections. We present a procedure to tomographically reconstruct the biodistribution of fluorescence in small animals. The imaging system and equipment are described, the step-by-step image acquisition and preliminary image-processing methods are presented, and the tomographic reconstruction procedure is outlined. Finally, the method is showcased by imaging the fluorescence activity of a brain tumor of a glioblastoma mouse model. © 2013 Cold Spring Harbor Laboratory Press.
APA:
Deliolanis, N.C., & Ntziachristos, V. (2013). Fluorescence molecular tomography of brain tumors in mice. Cold Spring Harbor Protocols, 8(5), 438-443. https://doi.org/10.1101/pdb.prot074245
MLA:
Deliolanis, Nikolaos C., and Vasilis Ntziachristos. "Fluorescence molecular tomography of brain tumors in mice." Cold Spring Harbor Protocols 8.5 (2013): 438-443.
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