Multimodal molecular imaging of integrin avb3 for in vivo detection of pancreatic cancer

Trajkovic-Arsic M, Mohajerani P, Sarantopoulos A, Kalideris E, Steiger K, Esposito I, Ma X, Themelis G, Burton N, Michalski CW, Kleeff J, Stangl S, Beer AJ, Pohle K, Wester HJ, Schmid RM, Braren R, Ntziachristos V, Siveke JT (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Journal of Nuclear Medicine Society of Nuclear Medicine

Book Volume: 55

Pages Range: 446-451

Journal Issue: 3

DOI: 10.2967/jnumed.113.129619

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease. Late detection of then nonresectable or metastasized tumors emphasizes the need for novel imaging approaches. Here, we report on so far nonexploited potentials of αvβ3 integrin-targeted molecular imaging technologies for detection of PDAC using genetically engineered mouse models. Methods: Immunohistochemistry and Western blot were used for characterization of αvβ3 expression in murine and human PDAC.We applied IntegriSense 680 fluorescencemolecular tomography, intraoperative fluorescence imaging, and ⁶⁸Ga-NODAGA-RGD PET for αvβ3 integrin molecular in vivo imaging of spontaneous PDAC occurring in Ptf1a^+/Cre;Kras ^{+/LSL-G12D;p53LoxP/LoxP} mice. (NODAGA is 1,4,7-triazacyclononane- 1,4-bis[acetic acid]-7-[2-glutaric acid] and RGD is arginine- glycine-aspartic acid.) Results: αvβ3 integrin is expressed in tumor cells of human and murine PDAC. IntegriSense fluorescence molecular tomography and ⁶⁸Ga-NODAGA-RGD PET enabled faithful visualization of PDAC. Furthermore, intraoperative optical imaging with IntegriSense 680 allowed good delineation of tumor borders. Conclusion: Imaging approaches targeting αvβ3 integrin expand the potential of molecular imaging for identification of αvβ3-positive PDAC with potential implications in early detection, fluorescenceguided surgery, and therapy monitoring. © 2014 by the Society of Nuclear Medicine and Molecular Imaging, Inc.

Involved external institutions

Technische Universität München (TUM)

Germany (DE) Technische Universität Dresden

Germany (DE)

How to cite

APA:

Trajkovic-Arsic, M., Mohajerani, P., Sarantopoulos, A., Kalideris, E., Steiger, K., Esposito, I.,... Siveke, J.T. (2014). Multimodal molecular imaging of integrin avb3 for in vivo detection of pancreatic cancer. Journal of Nuclear Medicine, 55(3), 446-451. https://doi.org/10.2967/jnumed.113.129619

MLA:

Trajkovic-Arsic, Marija, et al. "Multimodal molecular imaging of integrin avb3 for in vivo detection of pancreatic cancer." Journal of Nuclear Medicine 55.3 (2014): 446-451.

BibTeX: Download