Radiation-induced motility alterations in medulloblastoma cells

Rieken S, Rieber J, Brons S, Habermehl D, Rief H, Orschiedt L, Lindel K, Weber KJ, Debus J, Combs SE (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Journal of Radiation Research Oxford University Press (OUP): Policy C - Option B / Oxford University Press

Book Volume: 56

Pages Range: 430-436

Journal Issue: 3

DOI: 10.1093/jrr/rru120

Abstract

Photon irradiation has been repeatedly suspected of increasing tumor cell motility and promoting locoregional recurrence of disease. This study was set up to analyse possible mechanisms underlying the potentially radiation-altered motility in medulloblastoma cells. Medulloblastoma cell lines D425 and Med8A were analyzed in migration and adhesion experiments with and without photon and carbon ion irradiation. Expression of integrins was determined by quantitative FACS analysis. Matrix metalloproteinase concentrations within cell culture supernatants were investigated by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using Student's t-test. Both photon and carbon ion irradiation significantly reduced chemotactic medulloblastoma cell transmigration through 8-μm pore size membranes, while simultaneously increasing adherence to fibronectin- and collagen I- and IV-coated surfaces. Correspondingly, both photon and carbon ion irradiation downregulate soluble MMP9 concentrations, while upregulating cell surface expression of proadhesive extracellular matrix protein-binding integrin α5. The observed phenotype of radiation-altered motility is more pronounced following carbon ion than photon irradiation. Both photon and (even more so) carbon ion irradiation are effective in inhibiting medulloblastoma cell migration through downregulation of matrix metalloproteinase 9 and upregulation of proadhesive cell surface integrin α5, which lead to increased cell adherence to extracellular matrix proteins.

Involved external institutions

Universitätsklinikum Heidelberg DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Rieken, S., Rieber, J., Brons, S., Habermehl, D., Rief, H., Orschiedt, L.,... Combs, S.E. (2015). Radiation-induced motility alterations in medulloblastoma cells. Journal of Radiation Research, 56(3), 430-436. https://doi.org/10.1093/jrr/rru120

MLA:

Rieken, Stefan, et al. "Radiation-induced motility alterations in medulloblastoma cells." Journal of Radiation Research 56.3 (2015): 430-436.

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