Genetically Controlled Lysosomal Entrapment of Superparamagnetic Ferritin for Multimodal and Multiscale Imaging and Actuation with Low Tissue Attenuation

Massner C, Sigmund F, Pettinger S, Seeger M, Hartmann C, Ivleva NP, Niessner R, Fuchs H, De Angelis MH, Stelzl A, Koonakampully NL, Rolbieski H, Wiedwald U, Spasova M, Wurst W, Ntziachristos V, Winklhofer M, Westmeyer GG (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Advanced Functional Materials Wiley-VCH Verlag

Book Volume: 28

Article Number: 1706793

Journal Issue: 19

DOI: 10.1002/adfm.201706793

Abstract

Nanomaterials are of enormous value for biomedical applications because of their customizable features. However, the material properties of nanomaterials can be altered substantially by interactions with tissue thus making it important to assess them in the specific biological context to understand and tailor their effects. Here, a genetically controlled system is optimized for cellular uptake of superparamagnetic ferritin and subsequent trafficking to lysosomes. High local concentrations of photoabsorbing magnetoferritin give robust contrast in optoacoustic imaging and allow for selective photoablation of cells overexpressing ferritin receptors. Genetically controlled uptake of the biomagnetic nanoparticles also strongly enhances third-harmonic generation due to the change of refractive index caused by the magnetite–protein interface of ferritins entrapped in lysosomes. Selective uptake of magnetoferritin furthermore enables sensitive detection of receptor-expressing cells by magnetic resonance imaging, as well as efficient magnetic cell sorting and manipulation. Surprisingly, a substantial increase in the blocking temperature of lysosomally entrapped magnetoferritin is observed, which allows for specific ablation of genetically defined cell populations by local magnetic hyperthermia. The subcellular confinement of superparamagnetic ferritins thus enhances their physical properties to empower genetically controlled interrogation of cellular processes with deep tissue penetration.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Technische Universität München (TUM) DE Germany (DE) Universität Duisburg-Essen (UDE) DE Germany (DE) Carl von Ossietzky Universität Oldenburg DE Germany (DE)

How to cite

APA:

Massner, C., Sigmund, F., Pettinger, S., Seeger, M., Hartmann, C., Ivleva, N.P.,... Westmeyer, G.G. (2018). Genetically Controlled Lysosomal Entrapment of Superparamagnetic Ferritin for Multimodal and Multiscale Imaging and Actuation with Low Tissue Attenuation. Advanced Functional Materials, 28(19). https://doi.org/10.1002/adfm.201706793

MLA:

Massner, Christoph, et al. "Genetically Controlled Lysosomal Entrapment of Superparamagnetic Ferritin for Multimodal and Multiscale Imaging and Actuation with Low Tissue Attenuation." Advanced Functional Materials 28.19 (2018).

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