Visualizing cortical response to optogenetic stimulation and sensory inputs using multispectral handheld optoacoustic imaging

Ovsepian SV, Jiang Y, Sardella TCP, Malekzadeh-Najafabadi J, Burton NC, Yu X, Ntziachristos V (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Photoacoustics Elsevier

Book Volume: 17

Article Number: 100153

DOI: 10.1016/j.pacs.2019.100153

Abstract

To date, the vast majority of intra-vital neuroimaging systems applied in clinic and diagnostics is stationary with a rigid scanning element, requires specialized facilities and costly infrastructure. Here, we describe a simple yet radical approach for optoacoustic (photoacoustic) brain imaging in vivo using a light-weight handheld probe. It enables multispectral video-rate visualization of hemoglobin gradient changes in the cortex of adult rats induced by whisker and forelimb sensory inputs, as well as by optogenetic stimulation of intra-cortical connections. With superb penetration and molecular specificity, described here in method holds major promises for future applications in research, routine ambulatory neuroimaging, and diagnostics.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Max-Planck-Institut für biologische Kybernetik / Max Planck Institute for Biological Cybernetics DE Germany (DE) iThera Medical GmbH DE Germany (DE)

How to cite

APA:

Ovsepian, S.V., Jiang, Y., Sardella, T.C.P., Malekzadeh-Najafabadi, J., Burton, N.C., Yu, X., & Ntziachristos, V. (2020). Visualizing cortical response to optogenetic stimulation and sensory inputs using multispectral handheld optoacoustic imaging. Photoacoustics, 17. https://dx.doi.org/10.1016/j.pacs.2019.100153

MLA:

Ovsepian, Saak V., et al. "Visualizing cortical response to optogenetic stimulation and sensory inputs using multispectral handheld optoacoustic imaging." Photoacoustics 17 (2020).

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