A practical guide to photoswitching optoacoustics tomography
Stankevych M, Mishra K, Ntziachristos V, Stiel AC (2021)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2021
Journal
Publisher: Academic Press Inc.
Series: Methods in Enzymology
Book Volume: 657
Pages Range: 365-383
DOI: 10.1016/bs.mie.2021.06.031
Abstract
Photochromic proteins and photoswitching optoacoustics (OA) are a promising combination, that allows OA imaging of even small numbers of cells in whole live animals and thus can facilitate a more wide-spread use of OA in life-science and preclinical research. The concept relies on exploiting the modulation achieved by the photoswitching to discriminate the agents' signal from the non-modulating background. Here we share our analysis approaches that can be readily used on data generated with commercial OA tomography imaging instrumentation allowing—depending on the used photoswitching agent and sample—routine visualizations of as little as several hundreds of transgene labeled cells per imaging volume in the live animal.
Involved external institutions
Germany (DE)How to cite
APA:
Stankevych, M., Mishra, K., Ntziachristos, V., & Stiel, A.C. (2021). A practical guide to photoswitching optoacoustics tomography. In Jefferson Chan (Eds.), (pp. 365-383). Academic Press Inc..
MLA:
Stankevych, Mariia, et al. "A practical guide to photoswitching optoacoustics tomography." Ed. Jefferson Chan, Academic Press Inc., 2021. 365-383.
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