Characterization of Hormone Receptor and HER2 Status in Breast Cancer Using Mass Spectrometry Imaging

Gonçalves JPL, Bollwein C, Noske A, Jacob A, Jank P, Loibl S, Nekljudova V, Fasching P, Karn T, Marmé F, Müller V, Schem C, Sinn BV, Stickeler E, van Mackelenbergh M, Schmitt WD, Denkert C, Weichert W, Schwamborn K (2023)

Publication Type: Journal article

Publication year: 2023

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 24

Article Number: 2860

Journal Issue: 3

DOI: 10.3390/ijms24032860

Abstract

Immunohistochemical evaluation of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2 status stratify the different subtypes of breast cancer and define the treatment course. Triple-negative breast cancer (TNBC), which does not register receptor overexpression, is often associated with worse patient prognosis. Mass spectrometry imaging transcribes the molecular content of tissue specimens without requiring additional tags or preliminary analysis of the samples, being therefore an excellent methodology for an unbiased determination of tissue constituents, in particular tumor markers. In this study, the proteomic content of 1191 human breast cancer samples was characterized by mass spectrometry imaging and the epithelial regions were employed to train and test machine-learning models to characterize the individual receptor status and to classify TNBC. The classification models presented yielded high accuracies for estrogen and progesterone receptors and over 95% accuracy for classification of TNBC. Analysis of the molecular features revealed that vimentin overexpression is associated with TNBC, supported by immunohistochemistry validation, revealing a new potential target for diagnosis and treatment.

Authors with CRIS profile

Peter Fasching Professur für Translationale Frauenheilkunde und Geburtshilfe

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Universitätsklinikum Gießen und Marburg (UKGM) DE Germany (DE) GBG Forschungs GmbH (German Breast Group) DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE) Universitätsklinikum Mannheim DE Germany (DE) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Mammazentrum Hamburg MVZ GbR DE Germany (DE) Charité - Universitätsmedizin Berlin DE Germany (DE) Universitätsklinikum Aachen (UKA) DE Germany (DE) Universitätsklinikum Schleswig-Holstein (UKSH) DE Germany (DE)

How to cite

APA:

Gonçalves, J.P.L., Bollwein, C., Noske, A., Jacob, A., Jank, P., Loibl, S.,... Schwamborn, K. (2023). Characterization of Hormone Receptor and HER2 Status in Breast Cancer Using Mass Spectrometry Imaging. International Journal of Molecular Sciences, 24(3). https://doi.org/10.3390/ijms24032860

MLA:

Gonçalves, Juliana Pereira Lopes, et al. "Characterization of Hormone Receptor and HER2 Status in Breast Cancer Using Mass Spectrometry Imaging." International Journal of Molecular Sciences 24.3 (2023).

BibTeX: Download