A lipid membrane supported on an artificial extracellular matrix made of polyelectrolyte multilayers: towards nanoarchitectonics at the cellular interface
Vikulina A, Wulf A, Guday G, Fakhrullin R, Volodkin D (2022)
Publication Type: Journal article
Publication year: 2022
Journal
DOI: 10.1039/d2nr05186a
Abstract
To implement a specific function, cells recognize multiple physical and chemical cues and exhibit molecular responses at their interfaces - the boundary regions between the cell lipid-based membrane and the surrounding extracellular matrix (ECM). Mimicking the cellular external microenvironment presents a big challenge in nanoarchitectonics due to the complexity of the ECM and lipid membrane fragility. This study reports an approach for the assembly of a lipid bilayer, mimicking the cellular membrane, placed on top of a polyelectrolyte multilayer cushion made of hyaluronic acid and poly-l-lysine - a nanostructured biomaterial, which represents a 3D artificial ECM. Model proteins, lysozyme and alpha-lactalbumin, (which have similar molecular masses but carry opposite net charges) have been employed as soluble signalling molecules to probe their interaction with these hybrids. The formation of a lipid bilayer and the intermolecular interactions in the hybrid structure are monitored using a quartz crystal microbalance and confocal fluorescence microscopy. Electrostatic interactions between poly-l-lysine and the externally added proteins govern the transport of proteins into the hybrid. Designed ECM-cell mimicking hybrids open up new avenues for modelling a broad range of cell membranes and ECM and their associated phenomena, which can be used as a tool for synthetic biology and drug screening.
Authors with CRIS profile
Involved external institutions
Kazan (Volga region) Federal University / Казанский (Приволжский) федеральный университет
Russian Federation (RU)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
Nottingham Trent University
United Kingdom (GB)
Russian Federation (RU)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
Nottingham Trent University
United Kingdom (GB)
How to cite
APA:
Vikulina, A., Wulf, A., Guday, G., Fakhrullin, R., & Volodkin, D. (2022). A lipid membrane supported on an artificial extracellular matrix made of polyelectrolyte multilayers: towards nanoarchitectonics at the cellular interface. Nanoscale. https://dx.doi.org/10.1039/d2nr05186a
MLA:
Vikulina, Anna, et al. "A lipid membrane supported on an artificial extracellular matrix made of polyelectrolyte multilayers: towards nanoarchitectonics at the cellular interface." Nanoscale (2022).
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