Daum P, Ottmann S, Meinzinger J, Schulz S, Corte-Real J, Hauke M, Roth E, Schuh W, Mielenz D, Jäck HM, Pracht K (2022)
Publication Type: Journal article
Publication year: 2022
Book Volume: 13
DOI: 10.3389/fimmu.2022.991347
We have previously shown that the microRNA (miRNA) processor complex consisting of the RNAse Drosha and the DiGeorge Critical Region (DGCR) 8 protein is essential for B cell maturation. To determine whether miRNA processing is required to initiate T cell-mediated antibody responses, we deleted DGCR8 in maturing B2 cells by crossing a mouse with loxP-flanked DGCR8 alleles with a CD23-Cre mouse. As expected, non-immunized mice showed reduced numbers of mature B2 cells and IgG-secreting cells and diminished serum IgG titers. In accordance, germinal centers and antigen-specific IgG-secreting cells were absent in mice immunized with T-dependent antigens. Therefore, DGCR8 is required to mount an efficient T-dependent antibody response. However, DGCR8 deletion in B1 cells was incomplete, resulting in unaltered B1 cell numbers and normal IgM and IgA titers in DGCR8-knock-out mice. Therefore, this mouse model could be used to analyze B1 responses in the absence of functional B2 cells.
APA:
Daum, P., Ottmann, S., Meinzinger, J., Schulz, S., Corte-Real, J., Hauke, M.,... Pracht, K. (2022). The microRNA processing subunit DGCR8 is required for a T cell-dependent germinal center response. Frontiers in Immunology, 13. https://dx.doi.org/10.3389/fimmu.2022.991347
MLA:
Daum, Patrick, et al. "The microRNA processing subunit DGCR8 is required for a T cell-dependent germinal center response." Frontiers in Immunology 13 (2022).
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