Orofacial clefts alter early life oral microbiome maturation towards higher levels of potentially pathogenic species: A prospective observational study

Seidel CL, Strobel K, Weider M, Tschaftari M, Unertl C, Willershausen I, Weber M, Hörning A, Morhart P, Schneider M, Beckmann M, Bogdan C, Gerlach R, Gölz L (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Journal of Oral Microbiology Co-Action Publishing

Book Volume: 15

Article Number: 2164147

Journal Issue: 1

DOI: 10.1080/20002297.2022.2164147

Abstract

Orofacial clefts (OFC) present different phenotypes with a postnatal challenge for oral microbiota development. In order to investigate the impact of OFC on oral microbiota, smear samples from 15 neonates with OFC and 17 neonates without OFC were collected from two oral niches (tongue, cheek) at two time points, i.e. after birth (T0: Ø3d OFC group; Ø2d control group) and 4–5 weeks later (T1: Ø32d OFC group; Ø31d control group). Subsequently, the samples were analyzed using next-generation sequencing. We detected a significant increase of alpha diversity and anaerobic and Gram-negative species from T0 to T1 in both groups. Further, we found that at T1 OFC neonates presented a significantly lower alpha diversity (lowest values for high cleft severity) and significantly higher levels of Enterobacteriaceae (Citrobacter, Enterobacter, Escherichia-Shigella, Klebsiella), Enterococcus, Bifidobacterium, Corynebacterium, Lactocaseibacillus, Staphylococcus, Acinetobacter and Lawsonella compared to controls. Notably, neonates with unilateral and bilateral cleft lip and palate (UCLP/BCLP) presented similarities in beta diversity and a mixture with skin microbiota. However, significant differences were seen in neonates with cleft palate only compared to UCLP/BCLP with higher levels of anaerobic species. Our findings revealed an influence of OFC as well as cleft phenotype and severity on postnatal oral microbiota maturation.

Authors with CRIS profile

Karin Strobel Department of Orthodontics and Orofacial Orthopaedics Matthias Weider Department of Orthodontics and Orofacial Orthopaedics Marco Tschaftari Department of Orthodontics and Orofacial Orthopaedics Ines Willershausen Department of Orthodontics and Orofacial Orthopaedics Manuel Weber Department of Oral and Cranio-Maxillofacial Surgery André Hörning Department of Paediatrics and Adolescent Medicine Michael Schneider Department of Obstetrics and Gynaecology Matthias Beckmann Lehrstuhl für Geburtshilfe und Frauenheilkunde Christian Bogdan Lehrstuhl für Mikrobiologie und Infektionsimmunologie Roman Gerlach Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Lina Gölz Lehrstuhl für Zahn-, Mund- und Kieferheilkunde, insbesondere Kieferorthopädie

How to cite

APA:

Seidel, C.L., Strobel, K., Weider, M., Tschaftari, M., Unertl, C., Willershausen, I.,... Gölz, L. (2023). Orofacial clefts alter early life oral microbiome maturation towards higher levels of potentially pathogenic species: A prospective observational study. Journal of Oral Microbiology, 15(1). https://doi.org/10.1080/20002297.2022.2164147

MLA:

Seidel, Corinna Lesley, et al. "Orofacial clefts alter early life oral microbiome maturation towards higher levels of potentially pathogenic species: A prospective observational study." Journal of Oral Microbiology 15.1 (2023).

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