B cell fate mapping reveals their contribution to the memory immune response against helminths

Haase P, Schäfer S, Gerlach R, Winkler T, Vöhringer D (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Book Volume: 13

Article Number: 1016142

DOI: 10.3389/fimmu.2022.1016142

Abstract

An estimated quarter of the human world population is infected with gastrointestinal helminths causing major socioeconomic problems in endemic countries. A better understanding of humoral immune responses against helminths is urgently needed to develop effective vaccination strategies. Here, we used a fate mapping (FM) approach to mark germinal center (GC) B cells and their developmental fates by induced expression of a fluorescent protein during infection of mice with the helminth Nippostrongylus brasiliensis. We could show that FM⁺ cells persist weeks after clearance of the primary infection mainly as CD80⁺CD73⁺PD-L2⁺ memory B cells. A secondary infection elicited expansion of helminth-specific memory B cells and plasma cells (PCs). Adoptive transfers and analysis of somatic mutations in immunoglobulin genes further revealed that FM⁺ B cells rapidly convert to PCs rather than participating again in a GC reaction. These results provide new insights in the population dynamics of the humoral immune response against helminths.

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How to cite

APA:

Haase, P., Schäfer, S., Gerlach, R., Winkler, T., & Vöhringer, D. (2022). B cell fate mapping reveals their contribution to the memory immune response against helminths. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.1016142

MLA:

Haase, Paul, et al. "B cell fate mapping reveals their contribution to the memory immune response against helminths." Frontiers in Immunology 13 (2022).

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