Reliable determination of the main metabolites of 2-phenoxyethanol in human blood and urine using LC-MS/MS analysis

Jager T, Eckert E, Leibold E, Bader M (2022)


Publication Type: Journal article

Publication year: 2022

Journal

DOI: 10.1039/d2ay01407f

Abstract

2-Phenoxyethanol (PhE) is used as a broad-spectrum preservative in several consumer products like cosmetics and cleaning agents. To enable the analysis and assessment of human exposure to PhE, a fast and sensitive LC-MS/MS method for the quantification of two PhE metabolites, namely phenoxyacetic acid (PhAA) and 4-hydroxyphenoxyacetic acid (4-OH-PhAA) in human urine and blood was developed and validated. The method is based on liquid chromatography combined with tandem mass spectrometry (LC-MS/MS). Sample preparation was different for both matrices: either a simple "dilute&shoot"-approach for urine samples or a liquid-liquid-extraction (LLE) for blood samples was used. The limit of quantification (LOQ) is 10 mu g L-1 and 6 mu g L-1 for PhAA and 20 mu g L-1 and 10 mu g L-1 for 4-OH-PhAA in urine and blood, respectively. The method was applied to urine samples of 153 persons without occupational exposure to PhE and to blood samples of 7 additional volunteers. In blood, PhAA was detected in 57% of all samples (range:

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APA:

Jager, T., Eckert, E., Leibold, E., & Bader, M. (2022). Reliable determination of the main metabolites of 2-phenoxyethanol in human blood and urine using LC-MS/MS analysis. Analytical Methods. https://dx.doi.org/10.1039/d2ay01407f

MLA:

Jager, Thomas, et al. "Reliable determination of the main metabolites of 2-phenoxyethanol in human blood and urine using LC-MS/MS analysis." Analytical Methods (2022).

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