A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae

Imambocus BN, Formozov A, Zhou F, Soba P (2022)

Publication Type: Journal article

Publication year: 2022

Journal

STAR Protocols Cell Press

Book Volume: 3

Article Number: 101787

Journal Issue: 4

DOI: 10.1016/j.xpro.2022.101787

Abstract

Two-choice assays allow assessing of different behaviors including light avoidance in Drosophila larvae. Typically, the readout is limited to a preference index at a specific end point. We provide a detailed protocol to set up light avoidance assays and map the temporal distribution of larvae based on analysis of larval intensities. We describe the assay setup and implementation of scripts for analysis, which can be easily adapted to other two-choice assays and different model organisms. For complete details on the use and execution of this protocol, please refer to Imambocus et al. (2022).

Authors with CRIS profile

Peter Soba Professur für Zelluläre und Molekulare Neurophysiologie

Involved external institutions

Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE)

How to cite

APA:

Imambocus, B.N., Formozov, A., Zhou, F., & Soba, P. (2022). A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae. STAR Protocols, 3(4). https://dx.doi.org/10.1016/j.xpro.2022.101787

MLA:

Imambocus, Bibi Nusreen, et al. "A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae." STAR Protocols 3.4 (2022).

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