Chiral Protein-Covalent Organic Framework 3D-Printed Structures as Chiral Biosensors
Wang L, Gao W, Ng S, Pumera M (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 93
Pages Range: 5277-5283
Journal Issue: 12
DOI: 10.1021/acs.analchem.1c00322
Abstract
Three-dimensional (3D) printing technology has attracted great attention for prototyping different electrochemical sensor devices. However, chiral recognition remains a crucial challenge for electrochemical sensors with similar physicochemical properties such as enantiomers. In this work, a magnetic covalent organic framework (COF) and bovine serum albumin (BSA) (as the chiral surface) functionalized 3D-printed electrochemical chiral sensor is reported for the first time. The characterization of the chiral biomolecule-COF 3D-printed constructure was performed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and energy-dispersive X-ray spectroscopy (EDX). A tryptophan (Trp) enantiomer was chosen as the model chiral molecule to estimate the chiral recognition ability of the magnetic COF and BSA-based 3DE (Fe3O4@COF@BSA/3DE). We have demonstrated that the Fe3O4@COF@BSA/3DE exhibited excellent chiral recognition to l-Trp as compared to d-Trp. The chiral protein-COF sensing interface was used to determine the concentration of l-Trp in a racemic mixture of d-Trp and l-Trp. This strategy of on-demand fabrication of 3D-printed protein-COF-modified electrodes opens up new approaches for enantiomer recognition.
Authors with CRIS profile
Involved external institutions
Czech Republic (CZ)How to cite
APA:
Wang, L., Gao, W., Ng, S., & Pumera, M. (2021). Chiral Protein-Covalent Organic Framework 3D-Printed Structures as Chiral Biosensors. Analytical Chemistry, 93(12), 5277-5283. https://dx.doi.org/10.1021/acs.analchem.1c00322
MLA:
Wang, Lujun, et al. "Chiral Protein-Covalent Organic Framework 3D-Printed Structures as Chiral Biosensors." Analytical Chemistry 93.12 (2021): 5277-5283.
BibTeX: Download