Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing
Cho YK, Kim H, Benard A, Woo HK, Czubayko F, David P, Hansen F, Lee JI, Park JH, Schneck E, Weber G, Shin IS, Lee H (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 8
Pages Range: eabq4022-
Journal Issue: 38
Abstract
Electrochemiluminescence (ECL) has an inherently low background and enables precise chemical reactions through electrical control. Here, we report an advanced ECL system, termed ECLipse (ECL in paired signal electrode). We physically separated ECL generation from target detection: These two processes were carried out in isolated chambers and coupled through an electrode. The strategy allowed us to minimize cross-chemical reactions, design electrodes for high ECL signals, and integrate multiple sensors in a chip. As a proof of concept, we implemented an eight-plex ECLipse and applied it to detect host factors in human plasma. ECLipse achieved higher signal-to-noise ratio than conventional ECL assays and was >7000-fold more sensitive than enzyme-linked immunosorbent assay. In a pilot clinical study, we could detect septic conditions by measuring host factors [i.e., interleukin-3 (IL-3), IL-6, and procalcitonin (PCT)]. ECLipse assay further revealed distinct IL-3 and IL-6 patterns in patients with severe acute respiratory syndrome coronavirus 2 infection.
Authors with CRIS profile
Alan Benard
Department of Surgery
Franziska Czubayko
Department of Surgery
Frederik Hansen
Medizinische Fakultät
Georg Weber
Department of Surgery
Involved external institutions
Massachusetts General Hospital
United States (USA) (US)
Soongsil University
Korea, Republic of (KR)
Justus-Liebig-Universität Gießen
Germany (DE)
University of Massachusetts Lowell
United States (USA) (US)
Sogang University
Korea, Republic of (KR)
United States (USA) (US)
Soongsil University
Korea, Republic of (KR)
Justus-Liebig-Universität Gießen
Germany (DE)
University of Massachusetts Lowell
United States (USA) (US)
Sogang University
Korea, Republic of (KR)
How to cite
APA:
Cho, Y.K., Kim, H., Benard, A., Woo, H.K., Czubayko, F., David, P.,... Lee, H. (2022). Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing. Science Advances, 8(38), eabq4022-. https://dx.doi.org/10.1126/sciadv.abq4022
MLA:
Cho, Young Kwan, et al. "Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing." Science Advances 8.38 (2022): eabq4022-.
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