The Kaposi's sarcoma-associated herpesvirus-encoded vIRF-3 inhibits cellular IRF-5

Wies E, Hahn A, Schmidt K, Viebahn C, Rohland N, Lux A, Schellhorn T, Holzer A, Jung JU, Neipel F (2009)


Publication Type: Journal article

Publication year: 2009

Journal

Book Volume: 284

Pages Range: 8525-8538

Journal Issue: 13

DOI: 10.1074/jbc.M809252200

Abstract

Kaposi's sarcoma-associated herpesvirus encodes four genes with homology to the family of interferon regulatory factors (IRFs). At least one of these viral IRFs, vIRF-3, is expressed in latently Kaposi's sarcoma-associated herpesvirus-infected primary effusion lymphoma (PEL) cells and is essential for the survival of PEL cells. We now report that vIRF-3 interacts with cellular IRF-5, thereby inhibiting binding of IRF-5 to interferon-responsive promoter elements. Consequently, vIRF-3 blocked IRF-5-mediated promoter activation. A central double helix motif present in vIRF-3 was sufficient to abrogate both DNA binding and transcriptional transactivation by IRF-5. Upon DNA damage or activation of the interferon or Toll-like receptor pathways, cytoplasmic IRF-5 has been reported to be translocated to the nucleus, which results in induction of both p53-independent apoptosis and p21-mediated cell cycle arrest. We report here that IRF-5 is present in the nuclei of PEL cells without interferon stimulation. Silencing of vIRF-3 expression in PEL cells was accompanied by increased sensitivity to interferon- mediated apoptosis and up-regulation of IRF-5 target genes. In addition, vIRF-3 antagonized IRF-5-mediated activation of the p21 promoter. The data presented here indicate that vIRF-3 contributes to immune evasion and sustained proliferation of PEL cells by releasing IRF-5 from transcription complexes. © 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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APA:

Wies, E., Hahn, A., Schmidt, K., Viebahn, C., Rohland, N., Lux, A.,... Neipel, F. (2009). The Kaposi's sarcoma-associated herpesvirus-encoded vIRF-3 inhibits cellular IRF-5. Journal of Biological Chemistry, 284(13), 8525-8538. https://doi.org/10.1074/jbc.M809252200

MLA:

Wies, Effi, et al. "The Kaposi's sarcoma-associated herpesvirus-encoded vIRF-3 inhibits cellular IRF-5." Journal of Biological Chemistry 284.13 (2009): 8525-8538.

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