A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis

Ben Halima H, Bellagambi FG, Hangouët M, Alcacer A, Pfeiffer N, Heuberger A, Zine N, Bausells J, Elaissari A, Errachid A (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Talanta Elsevier

Book Volume: 251

Article Number: 123759

DOI: 10.1016/j.talanta.2022.123759

Abstract

Heart failure (HF) is a chronic cardiovascular disease that represents main cause of mortality worldwide, particularly for elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) was identified as the gold standard biomarker for HF diagnosis and therapy monitoring. Presently, saliva analysis represents an emerging and powerful tool for clinical applications and electrochemical immunosensors have shown their potential in Healthcare applications as selective and reliable systems for detecting clinical biomarkers. This work presents the detection of NT-proBNP in saliva samples by an immunologically modified Field effect Transistor (IMFET). TESUD ((11-triethoxysilyl) undecanal) was used as cross-linker to immobilise anti-NT-proBNP antibody onto the device. Our IMFET that was then tested in different matrices (e.g. phosphate buffered saline (PBS), artificial saliva and human saliva) using electrochemical impedance spectroscopy (EIS), and it resulted selective to NT-proBNP with good sensitivity (detection limit of 0.02 pg/mL) and a wide linear range (0.02–1 pg/mL and 0.5–20 pg/mL). Finally, NT-proBNP concentration in ten saliva samples was determined by performing the standard addition method. An enzyme-linked immunosorbent assay was used for confirming IMFET results, highlighting both IMFET accuracy (analyte recovery of 99 ± 8%) and precision (coefficient of variation always <10%), and supporting the suitability of the device for determining salivary NT-proBNP.

Authors with CRIS profile

Albert Heuberger Lehrstuhl für Informationstechnik mit dem Schwerpunkt Kommunikationselektronik (Stiftungslehrstuhl) (LIKE)

Additional Organisation(s)

FAU Profilzentrum Licht.Materie.Quantentechnologien (FAU LMQ)

Involved external institutions

Université Claude Bernard Lyon 1 (UCB) FR France (FR) National Center for Scientific Research / Centre national de la recherche scientifique (CNRS) FR France (FR) Institut de Microelectrònica de Barcelona (IMB-CNM) ES Spain (ES) Fraunhofer-Institut für Integrierte Schaltungen (IIS) DE Germany (DE)

How to cite

APA:

Ben Halima, H., Bellagambi, F.G., Hangouët, M., Alcacer, A., Pfeiffer, N., Heuberger, A.,... Errachid, A. (2023). A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis. Talanta, 251. https://doi.org/10.1016/j.talanta.2022.123759

MLA:

Ben Halima, Hamdi, et al. "A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis." Talanta 251 (2023).

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