Estrogens Determine Adherens Junction Organization and E-Cadherin Clustering in Breast Cancer Cells via Amphiregulin
Bischoff P, Kornhuber M, Dunst S, Zell J, Fauler B, Mielke T, Taubenberger A, Oelgeschlaeger M, Schoenfelder G, Guck J (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 23
Article Number: 101683
Journal Issue: 11
DOI: 10.1016/j.isci.2020.101683
Abstract
Estrogens play an important role in the development and progression of human cancers, particularly in breast cancer. Breast cancer progression depends on the malignant destabilization of adherens junctions (AJs) and disruption of tissue integrity. We found that estrogen receptor alpha (ERα) inhibition led to a striking spatial reorganization of AJs and microclustering of E-Cadherin (E-Cad) in the cell membrane of breast cancer cells. This resulted in increased stability of AJs and cell stiffness and a reduction of cell motility. These effects were actomyosin-dependent and reversible by estrogens. Detailed investigations showed that the ERα target gene and epidermal growth factor receptor (EGFR) ligand Amphiregulin (AREG) essentially regulates AJ reorganization and E-Cad microclustering. Our results not only describe a biological mechanism for the organization of AJs and the modulation of mechanical properties of cells but also provide a new perspective on how estrogens and anti-estrogens might influence the formation of breast tumors.
Authors with CRIS profile
Involved external institutions
Bundesinstitut für Risikobewertung (BfR)
Germany (DE)
Max-Planck-Institut für molekulare Genetik / Max Planck Institute for Molecular Genetics
Germany (DE)
Technische Universität Dresden
Germany (DE)
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
Germany (DE)
Max-Planck-Institut für molekulare Genetik / Max Planck Institute for Molecular Genetics
Germany (DE)
Technische Universität Dresden
Germany (DE)
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
How to cite
APA:
Bischoff, P., Kornhuber, M., Dunst, S., Zell, J., Fauler, B., Mielke, T.,... Guck, J. (2020). Estrogens Determine Adherens Junction Organization and E-Cadherin Clustering in Breast Cancer Cells via Amphiregulin. iScience, 23(11). https://doi.org/10.1016/j.isci.2020.101683
MLA:
Bischoff, Philip, et al. "Estrogens Determine Adherens Junction Organization and E-Cadherin Clustering in Breast Cancer Cells via Amphiregulin." iScience 23.11 (2020).
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