A monolithic glass chip for active single-cell sorting based on mechanical phenotyping

Faigle C, Lautenschlaeger F, Whyte G, Homewood P, Martin-Badosa E, Guck J (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Lab on A Chip Royal Society of Chemistry

Book Volume: 15

Pages Range: 1267-1275

Journal Issue: 5

DOI: 10.1039/c4lc01196a

Abstract

The mechanical properties of biological cells have long been considered as inherent markers of biological function and disease. However, the screening and active sorting of heterogeneous populations based on serial single-cell mechanical measurements has not been demonstrated. Here we present a novel monolithic glass chip for combined fluorescence detection and mechanical phenotyping using an optical stretcher. A new design and manufacturing process, involving the bonding of two asymmetrically etched glass plates, combines exact optical fiber alignment, low laser damage threshold and high imaging quality with the possibility of several microfluidic inlet and outlet channels. We show the utility of such a custom-built optical stretcher glass chip by measuring and sorting single cells in a heterogeneous population based on their different mechanical properties and verify sorting accuracy by simultaneous fluorescence detection. This offers new possibilities of exact characterization and sorting of small populations based on rheological properties for biological and biomedical applications.

Authors with CRIS profile

Jochen Guck

Involved external institutions

Technische Universität Dresden DE Germany (DE) University of Cambridge GB United Kingdom (GB) Blacktrace Holdings Ltd. GB United Kingdom (GB) Universitat de Barcelona (UB) / University of Barcelona ES Spain (ES)

How to cite

APA:

Faigle, C., Lautenschlaeger, F., Whyte, G., Homewood, P., Martin-Badosa, E., & Guck, J. (2015). A monolithic glass chip for active single-cell sorting based on mechanical phenotyping. Lab on A Chip, 15(5), 1267-1275. https://doi.org/10.1039/c4lc01196a

MLA:

Faigle, Christoph, et al. "A monolithic glass chip for active single-cell sorting based on mechanical phenotyping." Lab on A Chip 15.5 (2015): 1267-1275.

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