Wehrfritz A, Schmidt S, Ihmsen H, Schüttler J, Jeleazcov C (2022)
Publication Type: Journal article
Publication year: 2022
Book Volume: 2022
DOI: 10.1155/2022/3645048
The long-term stability of drugs under normal laboratory storage conditions (-20 degrees C) for years is important for research purposes, clinical re-evaluation, and also for forensic toxicology. To evaluate the stability of the analgesic opioid hydromorphone, 44 human frozen plasma samples of a former clinical trial were reanalyzed after at least three years. Blood samples were disposed using solid-phase extraction with an additional substitution of stable isotope labelled hydromorphone as an internal standard. Hydromorphone concentrations were determined by ultra-performance liquid chromatography (UPLC) with gradient elution, followed by tandem mass spectrometry with electrospray ionization. Calibration curves demonstrated linearity of the assay in the concentration range of 0.3-20 ng/mL hydromorphone. The limit of detection of the hydromorphone plasma concentration was 0.001 ng/mL, and the lower limit of quantification was 0.3 ng/mL. Intra- and interassay errors did not exceed 16%. The percentage deviation of the measured hydromorphone plasma concentrations between the reanalysis and the first analysis was -1.07% +/- 14.8% (mean +/- SD). These results demonstrate that hydromorphone concentration in human plasma was stable when the samples were frozen at -20 degrees C over three years. This finding is of value for re-evaluations or delayed analyses for research purposes and in pharmacokinetic studies, such as in forensic medicine.
APA:
Wehrfritz, A., Schmidt, S., Ihmsen, H., Schüttler, J., & Jeleazcov, C. (2022). Long-Term Stability of Hydromorphone in Human Plasma Frozen at-20 degrees C for Three Years Quantified by LC-MS/MS. International Journal of Analytical Chemistry, 2022. https://dx.doi.org/10.1155/2022/3645048
MLA:
Wehrfritz, Andreas, et al. "Long-Term Stability of Hydromorphone in Human Plasma Frozen at-20 degrees C for Three Years Quantified by LC-MS/MS." International Journal of Analytical Chemistry 2022 (2022).
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