Neurochemical classification of serotonin-immunoreactive neurons co-innervating motor endplates in the mouse esophagus

Zinsser-Krys J, Kapitza C, Böhnke L, van der Keylen P, Neuhuber W, Wörl J (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Abstract

Serotonin immunoreactivity was previously found in myenteric neurons co-innervating motor endplates in the mouse esophagus striated muscle and an involvement in motility control was suggested. However, it is not known if other neuroactive substances are present in these neurons and to what extent they co-localize. First, vasoactive intestinal peptide (VIP) was established as a bona fide marker for putative inhibitory myenteric neurons by evaluating co-localization with neuronal nitric oxide synthase (nNOS) and neuropeptide Y (NPY). Then, co-localization of serotonin and VIP was tested in co-innervating axons on motor endplates, which were visualized with α-bungarotoxin (α-BT) by multilabel immunofluorescence. Myenteric ganglia were also surveyed for co-localization in neuronal perikarya and varicosities. nNOS, NPY, and VIP were completely co-localized in enteric co-innervating nerve terminals on motor endplates. After co-staining with VIP, we found (a) serotonin (5-HT)-positive nerve endings without VIP (44% of 5-HT-positively innervated endplates), (b) 5-HT- and VIP-positive endings without co-localization (35%), and (c) 5-HT- and VIP-positive endings with co-localization (21%). About one-fifth of nerve terminals on motor endplates containing 5-HT originate from putative inhibitory peptidegic nitrergic neurons. However, the majority represents a different population presumably subserving different functions.

Authors with CRIS profile

Jillena Zinsser-Krys Lehrstuhl für Mikroskopische Anatomie und Molekulare Zellbiologie Christopher Kapitza Lehrstuhl für Mikroskopische Anatomie und Molekulare Zellbiologie Piet Keylen Lehrstuhl für Mikroskopische Anatomie und Molekulare Zellbiologie Winfried Neuhuber Lehrstuhl für Mikroskopische Anatomie und Molekulare Zellbiologie Jürgen Wörl Lehrstuhl für Mikroskopische Anatomie und Molekulare Zellbiologie

How to cite

APA:

Zinsser-Krys, J., Kapitza, C., Böhnke, L., van der Keylen, P., Neuhuber, W., & Wörl, J. (2022). Neurochemical classification of serotonin-immunoreactive neurons co-innervating motor endplates in the mouse esophagus. Anatomical Record. https://dx.doi.org/10.1002/ar.25030

MLA:

Zinsser-Krys, Jillena, et al. "Neurochemical classification of serotonin-immunoreactive neurons co-innervating motor endplates in the mouse esophagus." Anatomical Record (2022).

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