As in Real Estate, Location Matters: Cellular Expression of Complement Varies Between Macular and Peripheral Regions of the Retina and Supporting Tissues
Zauhar R, Biber J, Jabri Y, Kim M, Hu J, Kaplan L, Pfaller AM, Schaefer N, Enzmann V, Schlötzer-Schrehardt U, Straub T, Hauck SM, Gamlin PD, Mcferrin MB, Messinger J, Strang CE, Curcio CA, Dana N, Pauly D, Grosche A, Li M, Stambolian D (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 13
DOI: 10.3389/fimmu.2022.895519
Abstract
The cellular events that dictate the initiation of the complement pathway in ocular degeneration, such as age-related macular degeneration (AMD), is poorly understood. Using gene expression analysis (single cell and bulk), mass spectrometry, and immunohistochemistry, we dissected the role of multiple retinal and choroidal cell types in determining the complement homeostasis. Our scRNA-seq data show that the cellular response to early AMD is more robust in the choroid, particularly in fibroblasts, pericytes and endothelial cells. In late AMD, complement changes were more prominent in the retina especially with the expression of the classical pathway initiators. Notably, we found a spatial preference for these differences. Overall, this study provides insights into the heterogeneity of cellular responses for complement expression and the cooperation of neighboring cells to complete the pathway in healthy and AMD eyes. Further, our findings provide new cellular targets for therapies directed at complement.
Authors with CRIS profile
Involved external institutions
University City Science Center
United States (USA) (US)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
University of Pennsylvania
United States (USA) (US)
Universität Bern
Switzerland (CH)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
University of Alabama at Birmingham (UAB)
United States (USA) (US)
United States (USA) (US)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Universitätsklinikum Regensburg
Germany (DE)
University of Pennsylvania
United States (USA) (US)
Universität Bern
Switzerland (CH)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
University of Alabama at Birmingham (UAB)
United States (USA) (US)
How to cite
APA:
Zauhar, R., Biber, J., Jabri, Y., Kim, M., Hu, J., Kaplan, L.,... Stambolian, D. (2022). As in Real Estate, Location Matters: Cellular Expression of Complement Varies Between Macular and Peripheral Regions of the Retina and Supporting Tissues. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.895519
MLA:
Zauhar, Randy, et al. "As in Real Estate, Location Matters: Cellular Expression of Complement Varies Between Macular and Peripheral Regions of the Retina and Supporting Tissues." Frontiers in Immunology 13 (2022).
BibTeX: Download