Fabrication of Biocompatible Electrospun Poly(epsilon-caprolactone)/Gelatin Nanofibers Loaded with Pinus radiata Bark Extracts for Wound Healing Applications

Borges-Vilches J, Ünalan I, Fernandez K, Boccaccini AR (2022)


Publication Type: Journal article

Publication year: 2022

Journal

Book Volume: 14

Journal Issue: 12

DOI: 10.3390/polym14122331

Abstract

In this study, poly(epsilon-caprolactone) (PCL)/gelatin (GEL) electrospun nanofibers loaded with two different concentrations of Pinus radiata bark extracts (PEs) were fabricated via electrospinning for wound healing applications. The effects of incorporating PE into PCL/GEL electrospun nanofibers were investigated regarding their physicochemical properties and in vitro biocompatibility. All electrospun nanofibers showed smooth, uniform, and bead-free surfaces. Their functional groups were detected by ATR-FTIR spectroscopy, and their total phenol content was measured by a Folin-Ciocalteu assay. With PE addition, the electrospun nanofibers exhibited an increase in their wettability and degradation rates over time and a decrease in their tensile stress values from 20 +/- 4 to 8 +/- 2 MPa for PCL/GEL and PCL/GEL/0.36%PE samples, respectively. PE was also released from the fibrous mats in a rather controlled fashion. The PCL/GEL/0.18%PE and PCL/GEL/0.36%PE electrospun nanofibers inhibited bacterial activity at around 6 +/- 0.1% and 23 +/- 0.3% against E. coli and 14 +/- 0.1% and 18 +/- 0.2% against S. aureus after 24 h incubation, respectively. In vitro cell studies showed that PE-loaded electrospun nanofibers enhanced HaCaT cell growth, attachment, and proliferation, favoring cell migration towards the scratch area in the wound healing assay and allowing a complete wound closure after 72 h treatment. These findings suggested that PE-loaded electrospun nanofibers are promising materials for antibiotic-free dressings for wound healing applications.

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APA:

Borges-Vilches, J., Ünalan, I., Fernandez, K., & Boccaccini, A.R. (2022). Fabrication of Biocompatible Electrospun Poly(epsilon-caprolactone)/Gelatin Nanofibers Loaded with Pinus radiata Bark Extracts for Wound Healing Applications. Polymers, 14(12). https://doi.org/10.3390/polym14122331

MLA:

Borges-Vilches, Jessica, et al. "Fabrication of Biocompatible Electrospun Poly(epsilon-caprolactone)/Gelatin Nanofibers Loaded with Pinus radiata Bark Extracts for Wound Healing Applications." Polymers 14.12 (2022).

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