Magnetic drug targeting reduces the chemotherapeutic burden on circulating leukocytes

Janko C, Dürr S, Munoz LE, Lyer S, Chaurio R, Tietze R, Von Loehneysen S, Schorn C, Herrmann M, Alexiou C (2013)

Publication Type: Journal article

Publication year: 2013

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 14

Pages Range: 7341-7355

Journal Issue: 4

DOI: 10.3390/ijms14047341

Abstract

Magnetic drug targeting (MDT) improves the integrity of healthy tissues and cells during treatment with cytotoxic drugs. An anticancer drug is bound to superparamagnetic iron oxide nanoparticles (SPION), injected into the vascular supply of the tumor and directed into the tumor by means of an external magnetic field. In this study, we investigated the impact of SPION, mitoxantrone (MTO) and SPION^MTO on cell viability in vitro and the nonspecific uptake of MTO into circulating leukocytes in vivo. MDT was compared with conventional chemotherapy. MTO uptake and the impact on cell viability were assessed by flow cytometry in a Jurkat cell culture. In order to analyze MTO loading of circulating leukocytes in vivo, we treated tumor-bearing rabbits with MDT and conventional chemotherapy. In vitro experiments showed a dose-dependent MTO uptake and reduction in the viability and proliferation of Jurkat cells. MTO and SPION^MTO showed similar cytotoxic activity. Non-loaded SPION did not have any effect on cell viability in the concentrations tested. Compared with systemic administration in vivo, MDT employing SPION^MTO significantly decreased the chemotherapeutic load in circulating leukocytes. We demonstrated that MDT spares the immune system in comparison with conventional chemotherapy. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

Authors with CRIS profile

Christina Janko Einrichtungen, die zum Universitätsklinikum Erlangen gehören Stephan Dürr Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde Luis Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology Christoph Alexiou Professur für Nanomedizin

How to cite

APA:

Janko, C., Dürr, S., Munoz, L.E., Lyer, S., Chaurio, R., Tietze, R.,... Alexiou, C. (2013). Magnetic drug targeting reduces the chemotherapeutic burden on circulating leukocytes. International Journal of Molecular Sciences, 14(4), 7341-7355. https://doi.org/10.3390/ijms14047341

MLA:

Janko, Christina, et al. "Magnetic drug targeting reduces the chemotherapeutic burden on circulating leukocytes." International Journal of Molecular Sciences 14.4 (2013): 7341-7355.

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