The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations

Schicht M, Riedlova K, Kukulka M, Li W, Scheer A, Garreis F, Jacobi C, Paulsen F, Cwiklik L, Bräuer L (2022)

Publication Type: Journal article

Publication year: 2022

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 23

Journal Issue: 10

DOI: 10.3390/ijms23105783

Abstract

The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film.

Authors with CRIS profile

Martin Schicht Lehrstuhl für Funktionelle und Klinische Anatomie Wenyue Li Lehrstuhl für Funktionelle und Klinische Anatomie Fabian Garreis Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Lars Bräuer Professur für Oberflächenaktive Proteine

Involved external institutions

Academy of Sciences of the Czech Republic (ASCR) / Akademie věd České republiky (AVČR) CZ Czech Republic (CZ)

How to cite

APA:

Schicht, M., Riedlova, K., Kukulka, M., Li, W., Scheer, A., Garreis, F.,... Bräuer, L. (2022). The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations. International Journal of Molecular Sciences, 23(10). https://doi.org/10.3390/ijms23105783

MLA:

Schicht, Martin, et al. "The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations." International Journal of Molecular Sciences 23.10 (2022).

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