Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles
Friedrich RP, Kappes M, Cicha I, Tietze R, Braun C, Schneider-Stock R, Nagy R, Alexiou C, Janko C (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: Volume 17
Pages Range: 2139-2163
DOI: 10.2147/IJN.S355007
Abstract
Label-free detection of nanoparticles is essential for a thorough evaluation of their cellular effects. In particular, nanoparticles intended for medical applications must be carefully analyzed in terms of their interactions with cells, tissues, and organs. Since the labeling causes a strong change in the physicochemical properties and thus also alters the interactions of the particles with the surrounding tissue, the use of fluorescently labeled particles is inadequate to characterize the effects of unlabeled particles. Further, labeling may affect cellular uptake and biocompatibility of nanoparticles. Thus, label-free techniques have been recently developed and implemented to ensure a reliable characterization of nanoparticles.
This review provides an overview of frequently used label-free visualization techniques and highlights recent studies on the development and usage of microscopy systems based on reflectance, darkfield, differential interference contrast, optical coherence, photothermal, holographic, photoacoustic, total internal reflection, surface plasmon resonance, Rayleigh light scattering, hyperspectral and reflectance structured illumination imaging. Using these imaging modalities, there is a strong enhancement in the reliability of experiments concerning cellular uptake and biocompatibility of nanoparticles, which is crucial for preclinical evaluations and future medical applications.
Authors with CRIS profile
Iwona Cicha
Medizinische Fakultät
Rainer Tietze
Department of Otorhinolaryngology – Head and Neck Surgery
Regine Schneider-Stock
Professur für Experimentelle Tumorpathologie
Roland Nagy
Juniorprofessur für Elektronische Bauelemente
Christoph Alexiou
Professur für Nanomedizin
Christina Janko
Department of Otorhinolaryngology – Head and Neck Surgery
Additional Organisation(s)
Involved external institutions
Germany (DE)How to cite
APA:
Friedrich, R.P., Kappes, M., Cicha, I., Tietze, R., Braun, C., Schneider-Stock, R.,... Janko, C. (2022). Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles. International Journal of Nanomedicine, Volume 17, 2139-2163. https://doi.org/10.2147/IJN.S355007
MLA:
Friedrich, Ralf P., et al. "Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles." International Journal of Nanomedicine Volume 17 (2022): 2139-2163.
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