Hybrid Mucin-Vaterite Microspheres for Delivery of Proteolytic Enzyme Chymotrypsin
Balabushevich NG, Kovalenko EA, Filatova LY, Kirzhanova EA, Mikhalchik E, Volodkin D, Vikulina AS (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Abstract
While the enteral delivery of proteolytic enzymes is widely established for combating many diseases as an alternative to antibiotic treatment, their local delivery only emerges as administration route enabling sustained release in a controlled manner on site. The latest requires the development of drug delivery systems suitable for encapsulation and preservation of enzymatic proteolytic activity. This study proposes hybrid microspheres made of mucin and biodegradable porous crystals of calcium carbonate (CC) as the carriers for chymotrypsin (CTR) delivery. CTR is impregnated into CC and hybrid CC/mucin (CCM) microspheres by means of sorption without any chemical modification. The loading of the CC with mucin enhances CTR retention on hybrid microspheres (adsorption capacity of ≈8.7 mg g−1 vs 4.7 mg g−1), recharging crystal surface due to the presence of mucin and diminishing the average pore diameter of the crystals from 25 to 8 nm. Mucin also retards recrystallization of vaterite into nonporous calcite improving stability of CCM microspheres upon storage. Proteolytic activity of CTR is preserved in both CC and CCM microspheres, being pH dependent. Temperature-induced inactivation of CTR significantly diminishes by CTR encapsulation into CC and CCM microspheres. Altogether, these findings indicate promises of hybrid mucin-vaterite microspheres for mucosal application of proteases.
Involved external institutions
Federal Research & Clinical Center of Physical-Chemical Medicine / Федеральный научно-клинический центр физико-химической медицины
Russian Federation (RU)
Lomonosov Moscow State University / Московский государственный университет имени М.В.Ломоносова
Russian Federation (RU)
Russian Federation (RU)
Lomonosov Moscow State University / Московский государственный университет имени М.В.Ломоносова
Russian Federation (RU)
How to cite
APA:
Balabushevich, N.G., Kovalenko, E.A., Filatova, L.Y., Kirzhanova, E.A., Mikhalchik, E., Volodkin, D., & Vikulina, A.S. (2022). Hybrid Mucin-Vaterite Microspheres for Delivery of Proteolytic Enzyme Chymotrypsin. Macromolecular Bioscience. https://dx.doi.org/10.1002/mabi.202200005
MLA:
Balabushevich, Nadezhda G., et al. "Hybrid Mucin-Vaterite Microspheres for Delivery of Proteolytic Enzyme Chymotrypsin." Macromolecular Bioscience (2022).
BibTeX: Download