Dermal fillers do not induce upregulation of NLRP3 inflammasomes or expression of inflammatory cytokines in granulomas.

Reinholz M, Clanner-Engelshofen BM, Heppt MV, Marsela E, Kawakami Y, Wiest LG, French LE, Stolz W, Gauglitz GG (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Journal of Cosmetic Dermatology Blackwell Publishing Inc.

Book Volume: 19

Pages Range: 2838-2844

Journal Issue: 11

DOI: 10.1111/jocd.13341

Abstract

BACKGROUND: Filling materials have increasingly been used in aesthetics over the last decades. Understanding the pathophysiology of granuloma formation as a very relevant unwanted side effect of filler application may be essential to help avoid these adverse events. AIMS: Our aim was to investigate the role of the inflammasome in the formation of filler granuloma, as a central column of the innate immune response. METHODS: RPMI 1640 medium was used for growth of THP-1 cells and the induction of THP-1 macrophages. Sonication was applied in order to crush the acrylic particles of the filler. ELISA was the method of analysis for the specific cytokines. Biopsy specimens of filler granuloma were analyzed by various immunohistochemical methods. GraphPad Prism 5 software was used for the statistical data analysis. RESULTS: Neither was the sensor NALP3 overexpressed, nor could an elevated expression of cleaved IL-1β, IL-18, or IFN-γ be detected. Furthermore, no increased expression of IL-8 or IL-1β was detectable in vitro. CONCLUSION: No increased inflammasome activation could be observed; however, filler granulomas were infiltrated with granulocytes and macrophages. Therefore, we speculate that an unspecific immune response might be the key player in the formation of filler granuloma.

Involved external institutions

Ludwig-Maximilians-Universität (LMU) DE Germany (DE) Okayama University / 岡山大学 JP Japan (JP) München Klinik gGmbH DE Germany (DE)

How to cite

APA:

Reinholz, M., Clanner-Engelshofen, B.M., Heppt, M.V., Marsela, E., Kawakami, Y., Wiest, L.G.,... Gauglitz, G.G. (2020). Dermal fillers do not induce upregulation of NLRP3 inflammasomes or expression of inflammatory cytokines in granulomas. Journal of Cosmetic Dermatology, 19(11), 2838-2844. https://dx.doi.org/10.1111/jocd.13341

MLA:

Reinholz, Markus, et al. "Dermal fillers do not induce upregulation of NLRP3 inflammasomes or expression of inflammatory cytokines in granulomas." Journal of Cosmetic Dermatology 19.11 (2020): 2838-2844.

BibTeX: Download