Pleural Resident Macrophages and Pleural IRA B Cells Promote Efficient Immunity Against Pneumonia by Inducing Early Pleural Space Inflammation

Benard A, Podolska M, Czubayko F, Kutschick I, Klösch B, Jacobsen A, Naschberger E, Brunner M, Krautz C, Trufa D, Sirbu H, Lang R, Grützmann R, Weber G (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Frontiers in Immunology Frontiers Research Foundation / Frontiers Media

Book Volume: 13

Article Number: 821480

DOI: 10.3389/fimmu.2022.821480

Abstract

Airway infection is a major cause of mortality worldwide. The identification of new mechanisms aiding in effective host immune response is therefore required. Here, we show that the specific depletion of the pleural immune cell compartment during bacterial pneumonia resulted in a reduced pulmonary immune response and increased mortality in mice. Bacterial airway infection provoked early pleural space (PS) inflammation characterized by innate response activator (IRA) B cell development and pleural large resident macrophage (LRM) necroptosis, the repopulation of LRMs being driven by cellular proliferation in situ. Necroptotic LRMs amplified PS inflammation by stimulating pleural Mincle-expressing macrophages whereas IRA B cells contributed partially to GM-CSF-induced PS inflammation. Upon pulmonary infection, the induction of PS inflammation resulted in reduced bacterial burden whereas the specific depletion of pleural resident macrophages led to increased mortality and bacterial burden and reduced pulmonary immunity. Moreover, mice in which B cells were unable to produce GM-CSF exhibited reduced CD103⁺ dendritic cells and reduced CD4⁺ T cell numbers in the draining lymph node. Altogether, our results describe a previously unrecognized mechanism of pleural space inflammation necessary for effective protection against bacterial airway infection.

Authors with CRIS profile

Alan Benard Department of Surgery Malgorzata Podolska Department of Surgery Franziska Czubayko Department of Surgery Anne Jacobsen Department of Surgery Elisabeth Naschberger Department of Surgery Maximilian Brunner Medizinische Fakultät Christian Krautz Department of Surgery Denis Trufa Department of Thoracic Surgery Horia Sirbu Department of Thoracic Surgery Roland Lang Professur für Angeborene Immunität und Pathogenerkennung Robert Grützmann Department of Surgery Georg Weber Department of Surgery

How to cite

APA:

Benard, A., Podolska, M., Czubayko, F., Kutschick, I., Klösch, B., Jacobsen, A.,... Weber, G. (2022). Pleural Resident Macrophages and Pleural IRA B Cells Promote Efficient Immunity Against Pneumonia by Inducing Early Pleural Space Inflammation. Frontiers in Immunology, 13. https://dx.doi.org/10.3389/fimmu.2022.821480

MLA:

Benard, Alan, et al. "Pleural Resident Macrophages and Pleural IRA B Cells Promote Efficient Immunity Against Pneumonia by Inducing Early Pleural Space Inflammation." Frontiers in Immunology 13 (2022).

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