Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes-in-Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation

Wen P, Wang X, Moreno S, Boye S, Voigt D, Voit B, Huang X, Appelhans D (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Small Wiley-VCH Verlag

Book Volume: 17

Article Number: 2005749

Journal Issue: 7

DOI: 10.1002/smll.202005749

Abstract

The eukaryotic cell is a smart compartment containing an outer permeable membrane, a cytoskeleton, and functional organelles, presenting part structures for life. The integration of membrane-containing artificial organelles (=polymersomes) into a large microcompartment is a key step towards the establishment of exquisite cellular biomimetics with different membrane properties. Herein, an efficient way to construct a hierarchical multicompartment composed of a hydrogel-filled proteinosome hybrid structure with an outer homogeneous membrane, a smart cytoskeleton-like scaffold, and polymersomes is designed. Specially, this hybrid structure creates a micro-environment for pH-responsive polymersomes to execute a desired substance transport upon response to biological stimuli. Within the dynamic pH-stable skeleton of the protein hydrogels, polymersomes with loaded PEGylated insulin biomacromolecules demonstrate a pH-responsive reversible swelling-deswelling and a desirable, on-demand cargo release which is induced by the enzymatic oxidation of glucose to gluconic acid. This stimulus responsive behavior is realized by tunable on/off states through protonation of the polymersomes membrane under the enzymatic reaction of glucose oxidase, integrated in the skeleton of protein hydrogels. The integration of polymersomes-based hybrid structure into the proteinosome compartment and the stimuli-response on enzyme reactions fulfills the requirements of eukaryotic cell biomimetics in complex architectures and allows mimicking cellular transportation processes.

Involved external institutions

Harbin Institute of Technology (HIT) / 哈工大 CN China (CN) Leibniz-Institut für Polymerforschung Dresden e. V. DE Germany (DE) Technische Universität Dresden DE Germany (DE)

How to cite

APA:

Wen, P., Wang, X., Moreno, S., Boye, S., Voigt, D., Voit, B.,... Appelhans, D. (2021). Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes-in-Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation. Small, 17(7). https://doi.org/10.1002/smll.202005749

MLA:

Wen, Ping, et al. "Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes-in-Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation." Small 17.7 (2021).

BibTeX: Download