The construction and effect of physical properties on intracellular drug delivery of poly(amino acid) capsules
Huang W, Zhang T, Shi P, Yang D, Luo S, Voit B, Appelhans D, Zan X, Chen H (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 177
Pages Range: 178-187
DOI: 10.1016/j.colsurfb.2019.01.061
Abstract
Constructing intracellular degradable drug delivery vehicles is critical to fully exert the function of loaded drugs. Considering the poly (amino acid) is sensitively degradable to acid and enzyme which indwell in the mature lysosome, we here presented the poly(amino acid) capsules constructed by the synthetic poly(amino acid), (poly-glutamic acid, PGA and poly-ornithine, POR). The fabrication of Dox loaded poly (amino acid) capsules was demonstrated, and was thoroughly characterized by various techniques, including Zetasizer, SEM, TEM, fluorescent microscopy, and confocal laser scan microscopy. By controlling fabrication process, we tuned the carriers with different physical properties (charges and stiffness). Then, we thoroughly investigated the effects of these properties on the intracellular uptake and anti-cancer abilities of various carriers@Dox. In addition, the degradability of poly(amino acid) capsules was studied to reveal the release profiles of the carriers with or without templates from the side aspect. We found the positively charged and stiffer carriers mainly contributed to the cellular uptake process and amount, while both the uptake amount and degradability of the endocytosed carriers@Dox played a critical role on the cytotoxicity. We believe the findings here could pave the way for designing poly(amino acid) capsules or other degradable polymers based on poly(amino acid) as the drug delivery vehicles.
Involved external institutions
Wenzhou Medical University / 温州医科大学 (WMU)
China (CN)
Chinese Academy of Sciences (CAS) / 中国科学院
China (CN)
Leibniz-Institut für Polymerforschung Dresden e. V.
Germany (DE)
China (CN)
Chinese Academy of Sciences (CAS) / 中国科学院
China (CN)
Leibniz-Institut für Polymerforschung Dresden e. V.
Germany (DE)
How to cite
APA:
Huang, W., Zhang, T., Shi, P., Yang, D., Luo, S., Voit, B.,... Chen, H. (2019). The construction and effect of physical properties on intracellular drug delivery of poly(amino acid) capsules. Colloids and Surfaces B: Biointerfaces, 177, 178-187. https://dx.doi.org/10.1016/j.colsurfb.2019.01.061
MLA:
Huang, Wenjuan, et al. "The construction and effect of physical properties on intracellular drug delivery of poly(amino acid) capsules." Colloids and Surfaces B: Biointerfaces 177 (2019): 178-187.
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