Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process

Ccorahua R, Moreno S, Gumz H, Sahre K, Voit B, Appelhans D (2018)

Publication Type: Journal article

Publication year: 2018

Journal

RSC Advances Royal Society of Chemistry

Book Volume: 8

Pages Range: 25436-25443

Journal Issue: 45

DOI: 10.1039/c8ra03964j

Abstract

Reconstitution of biologically active polymersomes from the frozen or solid state into any fluid state is still a challenging issue for the design of new biological experiments and for the formulation of therapeutic agents. To gain knowledge about the reconstitution of pH-responsive and photo-crosslinked polymersomes, surface-functionalized and enzyme-containing polymersomers were cryogenically frozen (-20 °C) or freeze-dried with inulin as the lyoprotectant (0.1% w/v) and stored for a defined time period. Reconstituting those polymersomes in solution by thawing or a re-dispersing process revealed their original physical properties as well as their function as a pH-switchable enzymatic nanoreactor.

Involved external institutions

Pontificia Universidad Católica del Perú PE Peru (PE) Leibniz-Institut für Polymerforschung Dresden e. V. DE Germany (DE)

How to cite

APA:

Ccorahua, R., Moreno, S., Gumz, H., Sahre, K., Voit, B., & Appelhans, D. (2018). Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process. RSC Advances, 8(45), 25436-25443. https://dx.doi.org/10.1039/c8ra03964j

MLA:

Ccorahua, Robert, et al. "Reconstitution properties of biologically active polymersomes after cryogenic freezing and a freeze-drying process." RSC Advances 8.45 (2018): 25436-25443.

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