Quantitative analysis of BMP-2 derived peptide covalently grafted onto oxidized detonation nanodiamonds
Balakin S, Missirlis A, Klemmed B, Lee J, Opitz J, Yeo JS, Cuniberti G (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 152
Pages Range: 740-745
DOI: 10.1016/j.carbon.2019.05.064
Abstract
In order to deploy detonation nanodiamonds (NDs) in nanomedicine and drug delivery applications, fundamental understanding of their surface chemistry and drug loading capacity is highly desirable. Herein, bone morphogenetic protein 2 (BMP-2) derived peptide with the sequence KIPKASSVPTELSAISTLYLGGC (molecular weight = 2336 g/mol) have been successfully grafted onto NDs using carbodiimide crosslinker chemistry. Initially, functional surface groups of wet- and dry-oxidized NDs were compared using infrared and mass spectroscopy. Dry-oxidized NDs exhibited the highest amount of carboxylic acid derivatives with a surface loading of 0.113 mmol/g after air annealing at 415 °C for 5 h as determined by mass spectroscopy. Compared to wet-oxidation, the dry-oxidation process showed a 1.4-fold increased amount of carbonyl compounds deduced from infrared and mass spectroscopy. Crosslinking of the carboxylic acid derivatives to the primary amines of BMP-2 derived peptide is feasible in the range of 30–96% total surface coverage of NDs. 1:1 and 5:1 ND-peptide ratios were utilized to study the surface loading of NDs using fluorescence spectroscopy. The chemisorption of BMP-2 derived peptide onto NDs reveals superior surface coverage and results in reproducible amounts of tethered bioactive molecules.
Involved external institutions
Technische Universität Dresden
Germany (DE)
Fraunhofer-Institut für Keramische Technologien und Systeme (IKTS)
Germany (DE)
Yonsei University
Korea, Republic of (KR)
Germany (DE)
Fraunhofer-Institut für Keramische Technologien und Systeme (IKTS)
Germany (DE)
Yonsei University
Korea, Republic of (KR)
How to cite
APA:
Balakin, S., Missirlis, A., Klemmed, B., Lee, J., Opitz, J., Yeo, J.-S., & Cuniberti, G. (2019). Quantitative analysis of BMP-2 derived peptide covalently grafted onto oxidized detonation nanodiamonds. Carbon, 152, 740-745. https://doi.org/10.1016/j.carbon.2019.05.064
MLA:
Balakin, Sascha, et al. "Quantitative analysis of BMP-2 derived peptide covalently grafted onto oxidized detonation nanodiamonds." Carbon 152 (2019): 740-745.
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