S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water
Jung J, Lakatos M, Bengs S, Matys S, Raff J, Blueher A, Cuniberti G (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 183
Article Number: 110284
DOI: 10.1016/j.colsurfb.2019.06.014
Abstract
Bacterial surface layer proteins (S-layer) possess unique binding properties for metal ions. By combining the binding capability of S-layer proteins with the optical properties of gold nanoparticles (AuNP), namely plasmonic resonance, a colorimetric detection system for metal and metalloid ions in water was developed. Eight S-layer proteins from different bacteria species were used for the functionalization of AuNP. The thus developed biohybrid systems, AuNP functionalized with S-layer proteins, were tested with different metal salt solutions, e.g. Indium(III)-chloride, Yttrium(III)-chloride or Nickel(II)-chloride, to determine their selective and sensitive binding to ionic analytes. All tested S-layer proteins displayed unique binding affinities for the different metal ions. For each S-layer and metal ion combination markedly different reaction patterns and differences in concentration range and absorption spectra were detected by UV/vis spectroscopy. In this way, the selective detection of tested metal ions was achieved by differentiated analysis of a colorimetric screening assay of these biohybrid systems. A highly selective and sensitive detection of yttrium ions down to a concentration of 1.67 × 10−5 mol/l was achieved with S-layer protein SslA functionalized AuNP. The presented biohybrid systems can thus be used as a sensitive and fast sensor system for metal and metalloid ions in aqueous systems.
Involved external institutions
Technische Universität Dresden
Germany (DE)
University of Fribourg / Universitè de Fribourg
Switzerland (CH)
Universitätsspital Zürich (USZ)
Switzerland (CH)
Helmholtz-Zentrum Dresden-Rossendorf (HZDR)
Germany (DE)
Germany (DE)
University of Fribourg / Universitè de Fribourg
Switzerland (CH)
Universitätsspital Zürich (USZ)
Switzerland (CH)
Helmholtz-Zentrum Dresden-Rossendorf (HZDR)
Germany (DE)
How to cite
APA:
Jung, J., Lakatos, M., Bengs, S., Matys, S., Raff, J., Blueher, A., & Cuniberti, G. (2019). S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water. Colloids and Surfaces B: Biointerfaces, 183. https://dx.doi.org/10.1016/j.colsurfb.2019.06.014
MLA:
Jung, Jonas, et al. "S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water." Colloids and Surfaces B: Biointerfaces 183 (2019).
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