MyoBio: An automated bioreactor system technology for standardized perfusion-decellularization of whole skeletal muscle

Ritter P, Cai A, Reischl B, Fiedler M, Prölß G, Frie B, Kretzschmar E, Michael M, Hartmann K, Lesko C, Salti H, Arkudas A, Horch RE, Paulsen F, Friedrich O, Haug M (2022)

Publication Type: Journal article, Original article

Publication year: 2022

Journal

IEEE transactions on bio-medical engineering

DOI: 10.1109/TBME.2022.3142317

Abstract

Objective: Decellularizing solid organs is a promising top-down process to produce acellular bio-scaffolds for 'de novo' regrowth or application as tissue 'patches' that compensate, e.g., large volumetric muscle loss in reconstructive surgery. Therefore, generating standardized acellular muscle scaffolds marks a pressing area of need. Although animal muscle decellularization protocols were established, those are mostly manually performed and lack defined bioreactor environments and metrologies to assess decellularization quality in real-time. To close this gap, we engineered an automated bioreactor system to provide chemical decellularization solutions to immersed whole rat gastrocnemius medialis muscle through perfusion of the main feeding arteries. Results: Perfusion control is adjustable according to decellularization quality feedback. This was assessed both from (i) ex situ assessment of sarcomeres/nuclei through multiphoton fluorescence and label-free Second Harmonic Generation microscopy and DNA quantification, along with (ii) in situ assessment of the samples passive mechanical elasticity. Conclusion: We find DNA and sarcomere-free constructs after 72 h of 0.1% SDS perfusion-decellularization. Furthermore, passive elasticity can be implemented as additional online decellularization quality measure, noting a threefold elasticity decrease in acellular constructs. Significance: Our MyoBio represents a novel and useful automated bioreactor environment for standardized and controlled generation of acellular whole muscle scaffolds as a valuable source for regenerative medicine.

Authors with CRIS profile

Paul Ritter Lehrstuhl für Medizinische Biotechnologie Aijia Cai Department of Plastic and Hand Surgery Barbara Reischl Lehrstuhl für Medizinische Biotechnologie Gerhard Prölß Lehrstuhl für Medizinische Biotechnologie Elke Kretzschmar Lehrstuhl für Funktionelle und Klinische Anatomie Mena Michael Lehrstuhl für Medizinische Biotechnologie Kristin Hartmann Lehrstuhl für Medizinische Biotechnologie Christian Lesko Lehrstuhl für Medizinische Biotechnologie Andreas Arkudas Medizinische Fakultät Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie Michael Haug Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

Fraunhofer-Institut für Zelltherapie und Immunologie (IZI) DE Germany (DE)

How to cite

APA:

Ritter, P., Cai, A., Reischl, B., Fiedler, M., Prölß, G., Frie, B.,... Haug, M. (2022). MyoBio: An automated bioreactor system technology for standardized perfusion-decellularization of whole skeletal muscle. IEEE transactions on bio-medical engineering. https://doi.org/10.1109/TBME.2022.3142317

MLA:

Ritter, Paul, et al. "MyoBio: An automated bioreactor system technology for standardized perfusion-decellularization of whole skeletal muscle." IEEE transactions on bio-medical engineering (2022).

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