Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza A, Chang HD, Radbruch A, Abrignani S, Addo R, Akdis M, Andrä I, Andreata F, Annunziato F, Arranz E, Bacher P, Bari S, Barnaba V, Barros-Martins J, Baumjohann D, Beccaria CG, Bernardo D, Boardman DA, Borger J, Böttcher C, Brockmann L, Burns M, Busch DH, Cameron G, Cammarata I, Cassotta A, Chang Y, Chirdo FG, Christakou E, Čičin-Šain L, Cook L, Corbett AJ, Cornelis R, Cosmi L, Davey MS, De Biasi S, De Simone G, del Zotto G, Delacher M, Di Rosa F, Santo JD, Diefenbach A, Dong J, Dörner T, Dress RJ, Dutertre CA, Eckle SB, Eede P, Evrard M, Falk CS, Feuerer M, Fillatreau S, Fiz-Lopez A, Follo M, Foulds GA, Fröbel J, Gagliani N, Galletti G, Gangaev A, Garbi N, Garrote JA, Geginat J, Gherardin NA, Gibellini L, Ginhoux F, Godfrey DI, Gruarin P, Haftmann C, Hansmann L, Harpur CM, Hayday AC, Heine G, Hernández DC, Herrmann M, Hoelsken O, Huang Q, Huber S, Huber JE, Huehn J, Hundemer M, Hwang WY, Iannacone M, Ivison SM, Jäck HM, Jani PK, Keller B, Kessler N, Ketelaars S, Knop L, Knopf J, Koay HF, Kobow K, Kriegsmann K, Kristyanto H, Krueger A, Kuehne JF, Kunze-Schumacher H, Kvistborg P, Kwok I, Latorre D, Lenz D, Levings MK, Lino AC, Liotta F, Long HM, Lugli E, MacDonald KN, Maggi L, Maini MK, Mair F, Manta C, Manz RA, Mashreghi MF, Mazzoni A, McCluskey J, Mei HE, Melchers F, Melzer S, Mielenz D, Monin L, Moretta L, Multhoff G, Munoz LE, Muñoz-Ruiz M, Muscate F, Natalini A, Neumann K, Ng LG, Niedobitek A, Niemz J, Almeida LN, Notarbartolo S, Ostendorf L, Pallett LJ, Patel AA, Percin GI, Peruzzi G, Pinti M, Pockley AG, Pracht K, Prinz I, Pujol-Autonell I, Pulvirenti N, Quatrini L, Quinn KM, Radbruch H, Rhys H, Rodrigo MB, Romagnani C, Saggau C, Sakaguchi S, Sallusto F, Sanderink L, Sandrock I, Schauer C, Scheffold A, Scherer HU, Schiemann M, Schildberg FA, Schober K, Schoen J, Schuh W, Schüler T, Schulz AR, Schulz S, Schulze J, Simonetti S, Singh J, Sitnik KM, Stark R, Starossom S, Stehle C, Szelinski F, Tan L, Tarnok A, Tornack J, Tree TI, van Beek JJ, van de Veen W, van Gisbergen K, Vasco C, Verheyden NA, von Borstel A, Ward-Hartstonge KA, Warnatz K, Waskow C, Wiedemann A, Wilharm A, Wing J, Wirz O, Wittner J, Yang JH, Yang J (2021)


Publication Type: Journal article

Publication year: 2021

Journal

DOI: 10.1002/eji.202170126

Abstract

Abstract: The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples and respective applications of flow cytometry in the context of a variety of autoimmune diseases, cancers as well as acute and chronic infectious diseases such as COVID-19. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Authors with CRIS profile

Involved external institutions

Universität zu Lübeck DE Germany (DE) Deutsches Rheuma-Forschungszentrum (DRFZ) DE Germany (DE) University of Zurich / Universität Zürich (UZH) CH Switzerland (CH) Ospedale San Raffaele (früher: Centro San Raffaele del Monte Tabor Foundation) IT Italy (IT) Universidad de Valladolid ES Spain (ES) University of British Columbia CA Canada (CA) Institute for Research in Biomedicine CH Switzerland (CH) Christian-Albrechts-Universität zu Kiel DE Germany (DE) Technische Universität München (TUM) DE Germany (DE) Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy IT Italy (IT) Universität Leipzig DE Germany (DE) Humanitas Research Hospital / IRCCS Istituto Clinico Humanitas IT Italy (IT) Institution Nazional Genetica Molecolare Ingm IT Italy (IT) Goethe-Universität Frankfurt am Main DE Germany (DE) Università degli Studi di Modena e Reggio Emilia (UNIMORE) IT Italy (IT) Ludwig-Maximilians-Universität (LMU) DE Germany (DE) Universitätsklinikum Bonn DE Germany (DE) Universitätsmedizin der Johannes Gutenberg-Universität Mainz DE Germany (DE) Universitätsklinikum Freiburg DE Germany (DE) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut (FLI) DE Germany (DE) King’s College London GB United Kingdom (GB) Sanquin Diagnostic Services B.V. NL Netherlands (NL) Osaka University / 大阪大学 JP Japan (JP) Stanford University US United States (USA) (US) Helmholtz-Zentrum für Infektionsforschung (HZI) DE Germany (DE) Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital (NKI / NKI-AVL) NL Netherlands (NL) Otto-von-Guericke-Universität Magdeburg DE Germany (DE) Singapore Immunology Network (SIgN) SG Singapore (SG) Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich CH Switzerland (CH) Università degli Studi di Firenze / University of Florence IT Italy (IT) University of Birmingham GB United Kingdom (GB) BC Children's Hospital Research Institute CA Canada (CA) Fred Hutchinson Cancer Research Center US United States (USA) (US) Charité - Universitätsmedizin Berlin DE Germany (DE) Italian Institute of Technology / Istituto Italiano di Tecnologia (IIT) IT Italy (IT) RMIT University AU Australia (AU) The Francis Crick Institute GB United Kingdom (GB) Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE) Medizinische Hochschule Hannover (MHH) / Hannover Medical School DE Germany (DE) The University of Melbourne AU Australia (AU) Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Leiden University Medical Center NL Netherlands (NL) Ospedale Pediatrico Bambino Gesu IT Italy (IT) National Cancer Centre Singapore (NCCS) SG Singapore (SG) University College London (UCL) GB United Kingdom (GB) National Institute for Health and Medical Research / Institut national de la santé et de la recherche médicale (INSERM) FR France (FR) Nottingham Trent University GB United Kingdom (GB) Guy's and St Thomas' (NHS Foundation Trust) GB United Kingdom (GB) RCI - Regensburger Centrum für Interventionelle Immunologie DE Germany (DE) Università degli studi "La Sapienza" IT Italy (IT) University of Paris 5 - René Descartes / Université Paris V René Descartes FR France (FR) Universitätsklinikum Schleswig-Holstein (UKSH) DE Germany (DE) Columbia University US United States (USA) (US) National University of La Plata / Universidad Nacional de La Plata (UNLP) AR Argentina (AR) Istituto Giannina Gaslini IT Italy (IT) Institut Pasteur FR France (FR) Leibniz-Gemeinschaft DE Germany (DE) Hudson Institute of Medical Research AU Australia (AU) Duke-NUS Medical School / 杜克—国大医学研究生院 SG Singapore (SG)

How to cite

APA:

Cossarizza, A., Chang, H.D., Radbruch, A., Abrignani, S., Addo, R., Akdis, M.,... Yang, J. (2021). Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). European Journal of Immunology. https://doi.org/10.1002/eji.202170126

MLA:

Cossarizza, Andrea, et al. "Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)." European Journal of Immunology (2021).

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