Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza A, Chang HD, Radbruch A, Abrignani S, Addo R, Akdis M, Andrä I, Andreata F, Annunziato F, Arranz E, Bacher P, Bari S, Barnaba V, Barros-Martins J, Baumjohann D, Beccaria CG, Bernardo D, Boardman DA, Borger J, Böttcher C, Brockmann L, Burns M, Busch DH, Cameron G, Cammarata I, Cassotta A, Chang Y, Chirdo FG, Christakou E, Čičin-Šain L, Cook L, Corbett AJ, Cornelis R, Cosmi L, Davey MS, De Biasi S, De Simone G, del Zotto G, Delacher M, Di Rosa F, Santo JD, Diefenbach A, Dong J, Dörner T, Dress RJ, Dutertre CA, Eckle SB, Eede P, Evrard M, Falk CS, Feuerer M, Fillatreau S, Fiz-Lopez A, Follo M, Foulds GA, Fröbel J, Gagliani N, Galletti G, Gangaev A, Garbi N, Garrote JA, Geginat J, Gherardin NA, Gibellini L, Ginhoux F, Godfrey DI, Gruarin P, Haftmann C, Hansmann L, Harpur CM, Hayday AC, Heine G, Hernández DC, Herrmann M, Hoelsken O, Huang Q, Huber S, Huber JE, Huehn J, Hundemer M, Hwang WY, Iannacone M, Ivison SM, Jäck HM, Jani PK, Keller B, Kessler N, Ketelaars S, Knop L, Knopf J, Koay HF, Kobow K, Kriegsmann K, Kristyanto H, Krueger A, Kuehne JF, Kunze-Schumacher H, Kvistborg P, Kwok I, Latorre D, Lenz D, Levings MK, Lino AC, Liotta F, Long HM, Lugli E, MacDonald KN, Maggi L, Maini MK, Mair F, Manta C, Manz RA, Mashreghi MF, Mazzoni A, McCluskey J, Mei HE, Melchers F, Melzer S, Mielenz D, Monin L, Moretta L, Multhoff G, Munoz LE, Muñoz-Ruiz M, Muscate F, Natalini A, Neumann K, Ng LG, Niedobitek A, Niemz J, Almeida LN, Notarbartolo S, Ostendorf L, Pallett LJ, Patel AA, Percin GI, Peruzzi G, Pinti M, Pockley AG, Pracht K, Prinz I, Pujol-Autonell I, Pulvirenti N, Quatrini L, Quinn KM, Radbruch H, Rhys H, Rodrigo MB, Romagnani C, Saggau C, Sakaguchi S, Sallusto F, Sanderink L, Sandrock I, Schauer C, Scheffold A, Scherer HU, Schiemann M, Schildberg FA, Schober K, Schoen J, Schuh W, Schüler T, Schulz AR, Schulz S, Schulze J, Simonetti S, Singh J, Sitnik KM, Stark R, Starossom S, Stehle C, Szelinski F, Tan L, Tarnok A, Tornack J, Tree TI, van Beek JJ, van de Veen W, van Gisbergen K, Vasco C, Verheyden NA, von Borstel A, Ward-Hartstonge KA, Warnatz K, Waskow C, Wiedemann A, Wilharm A, Wing J, Wirz O, Wittner J, Yang JH, Yang J (2021)

Publication Type: Journal article

Publication year: 2021

Journal

DOI: 10.1002/eji.202170126

Abstract

Abstract: The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples and respective applications of flow cytometry in the context of a variety of autoimmune diseases, cancers as well as acute and chronic infectious diseases such as COVID-19. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Authors with CRIS profile

Involved external institutions

Universität zu Lübeck Germany (DE) Deutsches Rheuma-Forschungszentrum (DRFZ) Germany (DE) University of Zurich / Universität Zürich (UZH) Switzerland (CH) Ospedale San Raffaele (früher: Centro San Raffaele del Monte Tabor Foundation) Italy (IT) Universidad de Valladolid Spain (ES) University of British Columbia Canada (CA) Institute for Research in Biomedicine Switzerland (CH) Christian-Albrechts-Universität zu Kiel Germany (DE) Technische Universität München (TUM) Germany (DE) Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy Italy (IT) Universität Leipzig Germany (DE) Humanitas Research Hospital / IRCCS Istituto Clinico Humanitas Italy (IT) Institution Nazional Genetica Molecolare Ingm Italy (IT) Goethe-Universität Frankfurt am Main Germany (DE) Università degli Studi di Modena e Reggio Emilia (UNIMORE) Italy (IT) Ludwig-Maximilians-Universität (LMU) Germany (DE) Universitätsklinikum Bonn Germany (DE) Universitätsmedizin der Johannes Gutenberg-Universität Mainz Germany (DE) Universitätsklinikum Freiburg Germany (DE) Universitätsklinikum Hamburg-Eppendorf (UKE) Germany (DE) Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut (FLI) Germany (DE) King’s College London United Kingdom (GB) Sanquin Diagnostic Services B.V. Netherlands (NL) Osaka University / 大阪大学 Japan (JP) Stanford University United States (USA) (US) Helmholtz-Zentrum für Infektionsforschung (HZI) Germany (DE) Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital (NKI / NKI-AVL) Netherlands (NL) Otto-von-Guericke-Universität Magdeburg Germany (DE) Singapore Immunology Network (SIgN) Singapore (SG) Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich Switzerland (CH) Università degli Studi di Firenze / University of Florence Italy (IT) University of Birmingham United Kingdom (GB) BC Children's Hospital Research Institute Canada (CA) Fred Hutchinson Cancer Research Center United States (USA) (US) Charité - Universitätsmedizin Berlin Germany (DE) Italian Institute of Technology / Istituto Italiano di Tecnologia (IIT) Italy (IT) Royal Melbourne Institute of Technology (RMIT University) Australia (AU) The Francis Crick Institute United Kingdom (GB) Rheinische Friedrich-Wilhelms-Universität Bonn Germany (DE) Medizinische Hochschule Hannover (MHH) / Hannover Medical School Germany (DE) The University of Melbourne Australia (AU) Ruprecht-Karls-Universität Heidelberg Germany (DE) Leiden University Medical Center Netherlands (NL) Ospedale Pediatrico Bambino Gesu Italy (IT) National Cancer Centre Singapore (NCCS) Singapore (SG) University College London (UCL) United Kingdom (GB) National Institute for Health and Medical Research / Institut national de la santé et de la recherche médicale (INSERM) France (FR) Nottingham Trent University United Kingdom (GB) Guy's and St Thomas' (NHS Foundation Trust) United Kingdom (GB) RCI - Regensburger Centrum für Interventionelle Immunologie Germany (DE) Università degli studi "La Sapienza" Italy (IT) University of Paris 5 - René Descartes / Université Paris V René Descartes France (FR) Universitätsklinikum Schleswig-Holstein (UKSH) Germany (DE) Columbia University United States (USA) (US) National University of La Plata / Universidad Nacional de La Plata (UNLP) Argentina (AR) Istituto Giannina Gaslini Italy (IT) Institut Pasteur France (FR) Leibniz-Gemeinschaft Germany (DE) Hudson Institute of Medical Research Australia (AU) Duke-NUS Medical School / 杜克—国大医学研究生院 Singapore (SG)

How to cite

APA:

Cossarizza, A., Chang, H.D., Radbruch, A., Abrignani, S., Addo, R., Akdis, M.,... Yang, J. (2021). Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). European Journal of Immunology. https://doi.org/10.1002/eji.202170126

MLA:

Cossarizza, Andrea, et al. "Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)." European Journal of Immunology (2021).

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