Arabidopsis TANGLED Identifies the Division Plane throughout Mitosis and Cytokinesis

Walker KL, Müller S, Moss D, Ehrhardt DW, Smith LG (2007)

Publication Type: Journal article

Publication year: 2007


Book Volume: 17

Pages Range: 1827-1836

Journal Issue: 21

DOI: 10.1016/j.cub.2007.09.063


Background: In premitotic plant cells, the future division plane is predicted by a cortical ring of microtubules and F-actin called the preprophase band (PPB). The PPB persists throughout prophase, but is disassembled upon nuclear-envelope breakdown as the mitotic spindle forms. Following nuclear division, a cytokinetic phragmoplast forms between the daughter nuclei and expands laterally to attach the new cell wall at the former PPB site. A variety of observations suggest that expanding phragmoplasts are actively guided to the former PPB site, but little is known about how plant cells "remember" this site after PPB disassembly. Results: In premitotic plant cells, Arabidopsis TANGLED fused to YFP (AtTAN::YFP) colocalizes at the future division plane with PPBs. Strikingly, cortical AtTAN::YFP rings persist after PPB disassembly, marking the division plane throughout mitosis and cytokinesis. The AtTAN::YFP ring is relatively broad during preprophase/prophase and mitosis; narrows to become a sharper, more punctate ring during cytokinesis; and then rapidly disassembles upon completion of cytokinesis. The initial recruitment of AtTAN::YFP to the division plane requires microtubules and the kinesins POK1 and POK2, but subsequent maintenance of AtTAN::YFP rings appears to be microtubule independent. Consistent with the localization data, analysis of Arabidopsis tan mutants shows that AtTAN plays a role in guidance of expanding phragmoplasts to the former PPB site. Conclusions: AtTAN is implicated as a component of a cortical guidance cue that remains behind when the PPB is disassembled and directs the expanding phragmoplast to the former PPB site during cytokinesis. © 2007 Elsevier Ltd. All rights reserved.

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Walker, K.L., Müller, S., Moss, D., Ehrhardt, D.W., & Smith, L.G. (2007). Arabidopsis TANGLED Identifies the Division Plane throughout Mitosis and Cytokinesis. Current Biology, 17(21), 1827-1836.


Walker, Keely L., et al. "Arabidopsis TANGLED Identifies the Division Plane throughout Mitosis and Cytokinesis." Current Biology 17.21 (2007): 1827-1836.

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