Optimized analysis for sensitive detection and analysis of single proteins via interferometric scattering microscopy

Mirzaalian Dastjerdi H, Dahmardeh M, Gemeinhardt A, Mahmoodabadi RG, Köstler H, Sandoghdar V (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Journal of Physics D: Applied Physics IOP Publishing: Hybrid Open Access

Book Volume: 55

Journal Issue: 5

DOI: 10.1088/1361-6463/ac2f68

Abstract

It has been shown that interferometric detection of Rayleigh scattering (iSCAT) can reach an exquisite sensitivity for label-free detection of nano-matter, down to single proteins. The sensitivity of iSCAT detection is intrinsically limited by shot noise, which can be indefinitely improved by employing higher illumination power or longer integration times. In practice, however, a large speckle-like background and technical issues in the experimental setup limit the attainable signal-to-noise ratio. Strategies and algorithms in data analysis are, thus, crucial for extracting quantitative results from weak signals, e.g. regarding the mass (size) of the detected nano-objects or their positions. In this article, we elaborate on some algorithms for processing iSCAT data and identify some key technical as well as conceptual issues that have to be considered when recording and interpreting the data. The discussed methods and analyses are made available in the extensive python-based platform, PiSCAT(6).

Authors with CRIS profile

Houman Mirzaalian Dastjerdi Lehrstuhl für Informatik 10 (Systemsimulation) (LSS) Harald Köstler Lehrstuhl für Informatik 10 (Systemsimulation) (LSS) Vahid Sandoghdar Lehrstuhl für Experimentalphysik

Additional Organisation(s)

Lehrstuhl für Informatik 10 (Systemsimulation) (LSS)

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE)

How to cite

APA:

Mirzaalian Dastjerdi, H., Dahmardeh, M., Gemeinhardt, A., Mahmoodabadi, R.G., Köstler, H., & Sandoghdar, V. (2022). Optimized analysis for sensitive detection and analysis of single proteins via interferometric scattering microscopy. Journal of Physics D: Applied Physics, 55(5). https://doi.org/10.1088/1361-6463/ac2f68

MLA:

Mirzaalian Dastjerdi, Houman, et al. "Optimized analysis for sensitive detection and analysis of single proteins via interferometric scattering microscopy." Journal of Physics D: Applied Physics 55.5 (2022).

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