Method for Multiplexed Dynamic Intravital Multiphoton Imaging
Rakhymzhan A, Acs A, Leben R, Winkler T, Hauser AE, Niesner RA (2021)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2021
Journal
Publisher: Humana Press Inc.
Edited Volumes: Methods in Molecular Biology
Book Volume: 2350
Pages Range: 145-156
DOI: 10.1007/978-1-0716-1593-5_10
Abstract
Intravital two-photon microscopy enables monitoring of cellular dynamics and communication of complex systems, in genuine environment—the living organism. Particularly, its application in understanding the immune system brought unique insights into pathophysiologic processes in vivo. Here we present a method to achieve multiplexed dynamic intravital two-photon imaging by using a synergistic strategy combining a spectrally broad range of fluorophore emissions, a wave-mixing concept for simultaneous excitation of all targeted fluorophores, and an effective unmixing algorithm based on the calculation of spectral similarities with previously acquired fluorophore fingerprints. Our unmixing algorithm allows us to distinguish 7 fluorophore signals corresponding to various cellular and tissue compartments by using only four detector channels.
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Germany (DE)How to cite
APA:
Rakhymzhan, A., Acs, A., Leben, R., Winkler, T., Hauser, A.E., & Niesner, R.A. (2021). Method for Multiplexed Dynamic Intravital Multiphoton Imaging. In Methods in Molecular Biology. (pp. 145-156). Humana Press Inc..
MLA:
Rakhymzhan, Asylkhan, et al. "Method for Multiplexed Dynamic Intravital Multiphoton Imaging." Methods in Molecular Biology. Humana Press Inc., 2021. 145-156.
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