Spectroscopic characterization of the Co-substituted C-terminal domain of rubredoxin-2
Galle LM, Cutsail GE, Nischwitz V, Debeer S, Span I (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 399
Pages Range: 787-798
Journal Issue: 7
Abstract
Pseudomonas putida rubredoxin-2 (Rxn2) is an essential member of the alkane hydroxylation pathway and transfers electrons from a reductase to the membrane-bound hydroxylase. The regioselective hydroxylation of linear alkanes is a challenging chemical transformation of great interest for the chemical industry. Herein, we report the preparation and spectroscopic characterization of cobalt-substituted P. putida Rxn2 and a truncated version of the protein consisting of the C-terminal domain of the protein. Our spectroscopic data on the Co-substituted C-terminal domain supports a high-spin Co(II) with a distorted tetrahedral coordination environment. Investigation of the two-domain protein Rxn2 provides insights into the metal-binding properties of the N-terminal domain, the role of which is not well understood so far. Circular dichroism, electron paramagnetic resonance and X-ray absorption spectroscopies support an alternative Co-binding site within the N-terminal domain, which appears to not be relevant in nature. We have shown that chemical reconstitution in the presence of Co leads to incorporation of Co(II) into the active site of the C-terminal domain, but not the N-terminal domain of Rxn2 indicating distinct roles for the two rubredoxin domains.
Authors with CRIS profile
Involved external institutions
Heinrich-Heine-Universität Düsseldorf
Germany (DE)
Max-Planck-Institut für Chemische Energiekonversion (MPI CEC) / Max-Planck-Institute for Chemical Energy Conversion
Germany (DE)
Forschungszentrum Jülich / Research Centre Jülich (FZJ)
Germany (DE)
Germany (DE)
Max-Planck-Institut für Chemische Energiekonversion (MPI CEC) / Max-Planck-Institute for Chemical Energy Conversion
Germany (DE)
Forschungszentrum Jülich / Research Centre Jülich (FZJ)
Germany (DE)
How to cite
APA:
Galle, L.M., Cutsail, G.E., Nischwitz, V., Debeer, S., & Span, I. (2018). Spectroscopic characterization of the Co-substituted C-terminal domain of rubredoxin-2. Biological Chemistry, 399(7), 787-798. https://doi.org/10.1515/hsz-2018-0142
MLA:
Galle, Lisa M., et al. "Spectroscopic characterization of the Co-substituted C-terminal domain of rubredoxin-2." Biological Chemistry 399.7 (2018): 787-798.
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