The Adipose-Derived Stem Cell and Endothelial Cell Coculture System-Role of Growth Factors?

Steiner D, Mutschall H, Winkler S, Horch RE, Arkudas A (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Cells MDPI

Book Volume: 10

Journal Issue: 8

DOI: 10.3390/cells10082074

Abstract

Adequate vascularization is a fundamental prerequisite for bone regeneration, formation and tissue engineering applications. Endothelialization of scaffold materials is a promising strategy to support neovascularization and bone tissue formation. Besides oxygen and nutrition supply, the endothelial network plays an important role concerning osteogenic differentiation of osteoprogenitor cells and consecutive bone formation. In this study we aimed to enhance the growth stimulating, proangiogenic and osteogenic features of the ADSC and HUVEC coculture system by means of VEGFA165 and BMP2 application. We were able to show that sprouting phenomena and osteogenic differentiation were enhanced in the ADSC/HUVEC coculture. Furthermore, apoptosis was unidirectionally decreased in HUVECs, but these effects were not further enhanced upon VEGFA165 or BMP2 application. In summary, the ADSC/HUVEC coculture system per se is a powerful tool for bone tissue engineering applications.

Authors with CRIS profile

Dominik Steiner Medizinische Fakultät Hilkea Mutschall Medizinische Fakultät Sophie Winkler Department of Plastic and Hand Surgery Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Andreas Arkudas Medizinische Fakultät

How to cite

APA:

Steiner, D., Mutschall, H., Winkler, S., Horch, R.E., & Arkudas, A. (2021). The Adipose-Derived Stem Cell and Endothelial Cell Coculture System-Role of Growth Factors? Cells, 10(8). https://doi.org/10.3390/cells10082074

MLA:

Steiner, Dominik, et al. "The Adipose-Derived Stem Cell and Endothelial Cell Coculture System-Role of Growth Factors?" Cells 10.8 (2021).

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