Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice

Soumana IH, Linz B, Dewan KK, Sarr D, Gestal MC, Howard LK, Caulfield AD, Rada B, Harvill ET (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Emerging Infectious Diseases U.S. National Center for Infectious Diseases

Book Volume: 27

Pages Range: 2107-2116

Journal Issue: 8

DOI: 10.3201/eid2708.203566

Abstract

Conventional pertussis animal models deliver hundreds of thousands of Bordetella pertussis bacteria deep into the lungs, rapidly inducing severe pneumonic pathology and a robust immune response. However, human infections usually begin with colonization and growth in the upper respiratory tract. We inoculated only the nasopharynx of mice to explore the course of infection in a more natural exposure model. Nasopharyngeal colonization resulted in robust growth in the upper respiratory tract but elicited little immune response, enabling prolonged and persistent infection. Immunization with human acellular pertussis vaccine, which prevents severe lung infections in the conventional pneumonic infection model, had little effect on nasopharyngeal colonization. Our infection model revealed that B. pertussis can efficiently colonize the mouse nasopharynx, grow and spread within and between respiratory organs, evade robust host immunity, and persist for months. This experimental approach can measure aspects of the infection processes not observed in the conventional pneumonic infection model.

Involved external institutions

University of Georgia (UGA) US United States (USA) (US)

How to cite

APA:

Soumana, I.H., Linz, B., Dewan, K.K., Sarr, D., Gestal, M.C., Howard, L.K.,... Harvill, E.T. (2021). Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice. Emerging Infectious Diseases, 27(8), 2107-2116. https://dx.doi.org/10.3201/eid2708.203566

MLA:

Soumana, Illiassou Hamidou, et al. "Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice." Emerging Infectious Diseases 27.8 (2021): 2107-2116.

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