Modulation of the mucosa-associated microbiome linked to the PTPN2 risk gene in patients with primary Sclerosing cholangitis and ulcerative colitis

Denoth L, Juillerat P, Kremer AE, Rogler G, Scharl M, Yilmaz B, Bluemel S (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Microorganisms MDPI

Book Volume: 9

Article Number: 1752

Journal Issue: 8

DOI: 10.3390/microorganisms9081752

Abstract

Gutmicrobiota appears to be involved in the pathogenesis of primary sclerosing cholangitis (PSC). The protein tyrosine phosphatase nonreceptor 2 (PTPN2) gene risk variant rs1893217 is associated with gut dysbiosis in inflammatory bowel disease (IBD), and PTPN2 was mentioned as a possible risk gene for PSC. This study assessed the microbial profile of ulcerative colitis (UC) patients with PSC and without PSC (non-PSC). Additionally, effects of the PTPN2 risk variant were assessed. In total, 216 mucosal samples from ileum, colon, and rectum were collected from 7 PSC and 42 non-PSC patients, as well as 28 control subjects (non-IBD). The microbial composition was derived from 16S rRNA sequencing data. Overall, bacterial richness was highest in PSC patients, who also had a higher relative abundance of the genus Roseburia compared to non-PSC, as well as Haemophilus, Fusobacterium, Bifidobacterium, and Actinobacillus compared to non-IBD, as well as a lower relative abundance of Bacteroides compared to non-PSC and non-IBD, respectively. After exclusion of patients with the PTPN2 risk variant, Brachyspira was higher in PSC compared to non-PSC, while, solely in colon samples, Eubacterium and Tepidimonas were higher in PSC vs. non-IBD. In conclusion, this study underlines the presence of gut mucosa-associated microbiome changes in PSC patients and rather weakens the role of PTPN2 as a PSC risk gene.

Involved external institutions

Universitätsspital Zürich (USZ) CH Switzerland (CH) Universität Bern CH Switzerland (CH)

How to cite

APA:

Denoth, L., Juillerat, P., Kremer, A.E., Rogler, G., Scharl, M., Yilmaz, B., & Bluemel, S. (2021). Modulation of the mucosa-associated microbiome linked to the PTPN2 risk gene in patients with primary Sclerosing cholangitis and ulcerative colitis. Microorganisms, 9(8). https://doi.org/10.3390/microorganisms9081752

MLA:

Denoth, Luisa, et al. "Modulation of the mucosa-associated microbiome linked to the PTPN2 risk gene in patients with primary Sclerosing cholangitis and ulcerative colitis." Microorganisms 9.8 (2021).

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